Chromatographic analysis and pharmacokinetics of liposome-encapsulated doxorubicin in non-small-cell lung cancer patients.

A sensitive and specific quantitative assay for total doxorubicin concentrations in plasma containing liposome-encapsulated doxorubicin hydrochloride (TLC D-99) was developed, with solvent extraction and reversed-phase high-performance liquid chromatography (HPLC). Separation of doxorubicin from its metabolites was accomplished with a 15 cm x 3.9 mm i.

HPLC assay for guanidine salts based on pre-column derivatization with acetylacetone.

A specific, isocratic HPLC assay procedure for guanidine hydrochloride is developed. A key step in the procedure, pre-column derivatization with acetylacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out the derivatization are given and the effects of reaction variables are discussed.