Morphokinetic Analyses of Fishing Cat-Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System.

A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. The objective of this study was to investigate the morphokinetic parameters of fishing cat-domestic cat interspecies somatic cell nuclear transfer (iSCNT) embryos from one-cell to blastocyst stages, and in particular, the cleavage patterns of the first division in iSCNT and IVF embryos, as these play a central role in euploidy.

EZH2 modulates mRNA splicing and exerts part of its oncogenic function through repression of splicing factors in CML.

The polycomb protein EZH2 is up-regulated in Chronic Myeloid Leukaemia (CML) and associated with transcriptional reprogramming. Here we tested whether EZH2 might also act as a modulator of the mRNA splicing landscape to elicit its oncogenic function in CML. We treated CML cell lines with EZH2 inhibitors and detected differential splicing of several hundreds of events, potentially caused by the transcriptional regulation of splicing factors.

Geocultural differences in pre-schooler sleep profiles and family practices: an analysis of pooled data from 37 countries.

Study Objectives: To examine 1) multidimensional sleep profiles in pre-schoolers (3-6 years) across geocultural regions and 2) differences in sleep characteristics and family practices between Majority World regions (Pacific Islands, Sub-Saharan Africa, Eastern Europe, Northeast Asia, Southeast Asia, South Asia, the Middle East and North Africa, Latin America) and the Minority World (the Western world).

Methods: Participants were 3507 pre-schoolers from 37 countries. Nighttime sleep characteristics and nap duration (accelerometer: n=1950) and family practices (parental questionnaire) were measured.

Effects of open-skill and closed-skill exercise on subthreshold depression in female adolescents: A randomized controlled trial.

Background: Subthreshold depression (SD) affects a significant proportion of adolescent females, posing a risk of major depression in later life. This study examines the effects of open-skill exercise (OSE) and closed-skill exercise (CSE) on SD, executive function (EF), and emotional states in female adolescents.

Methods: A double-blind randomized controlled trial involved 95 female adolescents (mean age = 16.

Disruption of bioenergetics enhances the radio-sensitivity of patient-derived glioblastoma tumorspheres.

Background: Despite available treatment approaches, including surgical resection along with chemotherapy and radiotherapy, glioblastoma (GBM), the most prevalent primary brain tumor, remains associated with a grim prognosis. Although radiotherapy is central to GBM treatment, its combination with bioenergetics regulators has not been validated in clinical practice. Here, we hypothesized that bioenergetics regulators can enhance the radio-sensitivity of GBM tumorspheres (TSs).