We have investigated the use of DualEELS in elementally sensitive tilt series tomography in the scanning transmission electron microscope. A procedure is implemented using deconvolution to remove the effects of multiple scattering, followed by normalisation by the zero loss peak intensity. This is performed to produce a signal that is linearly dependent on the projected density of the element in each pixel.
View Article and Find Full Text PDFElectron tomography (ET) is an increasingly important technique for the study of the three-dimensional morphologies of nanostructures. ET involves the acquisition of a set of two-dimensional projection images, followed by the reconstruction into a volumetric image by solving an inverse problem. However, due to limitations in the acquisition process, this inverse problem is ill-posed (i.
View Article and Find Full Text PDFThe three-dimensional (3-D) pyramid compressor project at the University of Glasgow has developed a compressor for images obtained from confocal laser scanning microscopy (CLSM) device. The proposed method using a combination of image pyramid coder and vector quantization techniques has good performance at compressing confocal volume image data. An experiment was conducted on several kinds of CLSM data using the presented compressor compared with other well-known volume data compressors, such as MPEG-1.
View Article and Find Full Text PDFBackground: Symptomatic osteopenia is a common form of morbidity in children with acute lymphoblastic leukemia (ALL) before, during, and after treatment. A causal role for corticosteroids has been proposed, but other investigators have suggested that cranial irradiation is an important factor contributing to this disorder.
Procedure: In this study of children with ALL, all of whom received steroids, skeletal morbidity was assessed radiographically by an observer who was blinded to the ages of the children, their risk categorization (and related treatment), and the timing of the assessments with respect to the administration of therapy.
Objective: To determine whether the osteopenia and unusual fractures observed in children with acute lymphoblastic leukemia (ALL) were related to the disease rather than to its treatment.
Design: Prospective analysis of the bone and mineral status in 40 consecutive children with ALL seen in a pediatric tertiary-care referral center.
Methods: Biochemical indicators of mineral, endocrine, and vitamin D status were measured before initiation of therapy.