Effect of obesity on outcomes after breast reconstruction surgery, an analysis of national surgical quality improvement program.

Background: Obesity is a risk factor for breast cancer and may affect the incidence, and outcomes of surgical treatment for breast cancer, including breast reconstruction.

Objective: This study aimed to evaluate outcomes of breast reconstruction in patients with obesity.

Methods: In a retrospective review of the NSQIP 2013-2018, adult patients who underwent breast reconstruction were included.

Changes in order parameters associated with ceramide-mediated membrane reorganization measured using pTIRFM.

The enzymatic generation of ceramide has significant effects on the biophysical properties of lipid bilayers and can lead to the extensive reorganization of cell membranes. We have synthesized and characterized a headgroup-labeled fluorescent lipid probe (NBD-ceramide, NBD-Cer) and demonstrated that it can be used for polarized total internal reflection fluorescence microscopy experiments to probe changes in membrane order that result from ceramide incorporation. NBD-Cer measures significantly higher order parameters for the liquid-ordered (Lo) domains ([P2] = 0.