Behavior of endothelial cells cultured on Silastic and Dacron velour under flow conditions in vitro: implications for prelining vascular grafts with cells.

Tissue-cultured bovine aortic endothelial cells were subjected to flow in an in vitro circulatory loop designed to simulate the flow and pressure conditions in the aorta. The cells were cultured under stationary conditions on tubes of fibronectin-coated Silastic or of Dacron velour. The tubes were then added to the flow loop, which circulated complete tissue culture medium in a pulsatile mode.

In vitro evaluation of U.S. and U.S.S.R. artificial hearts.

This report summarizes the results of the in vitro evaluation of Soviet and American artificial hearts. The devices were tested at the All-Union Institute of Transplantation and Artificial Organs, Moscow, U.S.

Performance of tissue cultured endothelial cells in a mock circulatory loop.

Tissue cultured bovine aortic endothelial cells were subjected to flow in an in vitro circulatory loop which was designed to simulate the flow and pressure conditions in the aorta. The cells were cultured to confluence under stationary conditions on a tube consisting of microfabric backed with polyurethane. The tube with the cultured cells was then added to the flow loop which circulated complete tissue culture medium in a pulsatile mode.

A second generation portable life support system.

Recognizing the need to transport critically ill patients to a specialized facility, we have designed a profound life support system. PLSS addresses the demand for mobility by placing all support systems under a patient cart. It addresses the demand for reliable power supply by providing a redundancy in sources.