Publications by authors named "W Nguyen"
Introduction: Cutaneous T-cell lymphoma (CTCL) is closely associated with the host microbiome. While recent evidence suggests that shifts in specific bacterial taxa are associated with response to UV-B, a form of non-ionizing radiation, the impact of ionizing radiation (IR) has not been investigated.
Methods: 16S rRNA and gene amplicon sequencing were performed on DNA extracted from swabs of lesional/non-lesional skin of 12 CTCL patients before/after TSEBT or local IR and from 25 matched healthy controls (HC).
Article Synopsis
- SARS-CoV-2, responsible for COVID-19, can cause severe disease characterized by inflammatory responses like cytokine storms, but many infections are mild or asymptomatic.
- Researchers conducted RNA-Seq and histological analyses on mouse lungs infected with the omicron BA.1 variant, finding that while robust infection occurred initially, the virus was mostly cleared by day 10 post-infection.
- Acute inflammatory responses showed notably different cytokine patterns compared to severe cases, with an increase in anti-inflammatory cell types and a trend towards recovery, indicating that the immune response could foster "protective inflammation" leading to recovery without severe sequelae.
Angiotensin-converting enzyme 2 (ACE2) is the primary entry receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but ACE2-independent entry has been observed in vitro for strains with the spike-E484D substitution. Here, we conduct a whole-genome CRISPR-Cas9 knockout screen using SARS-CoV-2 mouse adapted 1 (SARS-CoV-2), which carries spike-E484D, to identify the ACE2-independent entry mechanisms. SARS-CoV-2 infection in HEK293T cells relies on heparan sulfate and endocytic pathways, with TMEM106B, a transmembrane lysosomal protein, the most significant contributor.
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- Drug resistance is making existing antimalarials ineffective, highlighting the urgent need for new treatments.
- Researchers identified a promising new chemotype, cyclopropyl carboxamide, through screening a library of compounds, leading to the development of a strong candidate, WJM280, which is effective against malaria without harming human cells.
- Further studies revealed that resistant parasites have mutations in the cytochrome b gene, confirming it as the drug target, but improving the compound's stability and effectiveness in mouse models still needs to be addressed.