Publications by authors named "W Milestone"
Article Synopsis
- - This study examines how complex cellular structures, specifically Jurkat cells with features like the endoplasmic reticulum and mitochondria, respond to external electric pulses by modeling transmembrane potential and electroporation.
- - Simulations reveal that electroporation requires a stronger electric field for the endoplasmic reticulum compared to the inner mitochondrial membrane, which is more susceptible to poration, and may be linked to increased intracellular calcium levels.
- - The research also explores how repeated electric pulses and electrode placement enhance membrane poration, while acknowledging that although more complex models could improve accuracy, basic trends in findings are likely to persist.
Maps of Membrane Pore Dynamics From Picosecond to Millisecond Pulse Durations.
IEEE Trans Biomed Eng
September 2024
Electroporation occurs when cells are exposed to an electric pulse of sufficient intensity E and pulse duration τ. Many studies have attempted to develop universal scaling laws to predict membrane pore dynamics for pulsed electric fields (PEFs) of different durations; however, the differences in pore dynamics across these parameters makes this difficult both experimentally and numerically. This study uses the asymptotic Smoluchowski equation (ASME) to quantify the number of pores, average pore radius, and fractional pore area (FPA) during exposure to PEFs with durations from hundreds of picoseconds to a millisecond.
View Article and Find Full Text PDFSelf-consistent evaluations of membrane electroporation along with local heating in single spherical cells arising from external AC radiofrequency electrical stimulation have been carried out. The present numerical study seeks to determine whether healthy and malignant cells exhibit separate electroporative responses with regards to operating frequency. It is shown that cells of Burkitt's lymphoma would respond to frequencies >4.
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