Minimizing Unnecessary Harvesting of an Extremely Thin Gracilis Tendon During ACL Reconstruction by Referencing the Diameter of the Doubled Semitendinosus Tendon Alone.

Background: The incidence of anterior cruciate ligament reconstruction (ACLR) graft failure is inversely related to the diameter of the ACLR graft. When the diameter of a 4-stranded hamstring tendon graft with a doubled semitendinosus and doubled gracilis tendon (ST×2 + G×2) configuration is <8 mm, the gracilis tendon is often thin.

Hypothesis: (1) The diameter of the doubled semitendinosus tendon (ST×2) alone would be able to predict the probability of a 4-stranded ACLR graft having a diameter of ≥8 mm, and (2) there would be a specific cutoff value for the ST×2 diameter such that the addition of a doubled gracilis tendon (G×2) will not result in a 4-stranded graft with a ≥8-mm diameter.

Sex-Specific Vulnerabilities to Subclinical Vascular Brain Injury in Early Late-Life: The Framingham Heart Study.

Objective: Subclinical vascular brain injury is an increasingly recognized risk factor for stroke and dementia. Despite well-established sex differences in vascular risk and disease prevalence, the impact of sex on drivers of subclinical vascular brain injury remains unclear, presenting a barrier to developing sex-specific prevention guidelines. We aimed to establish the extent to which sex moderates associations between vascular risk factors and magnetic resonance imaging (MRI) measures of subclinical brain injury in stroke-free older adults.

Comparison of Medial Hamstring Length Between Harvesting Methods Using Anterior and Posterior Approaches.

Background: Medial hamstring tendons harvesting can be performed through either an anterior or a posterior approach. It has been suggested that using a posterior approach may result in a longer length of harvestable tendon compared with an anterior approach.

Hypothesis: There would be no difference in the length of the harvestable tendon between the anterior and posterior approaches.

Conformations of a low-complexity protein in homogeneous and phase-separated frozen solutions.

Solutions of the intrinsically disordered, low-complexity domain of the FUS protein (FUS-LC) undergo liquid-liquid phase separation (LLPS) below a temperature T. To investigate whether local conformational distributions are detectably different in the homogeneous (i.e.

Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation.

Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum (ER) membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we imaged tick-borne flavivirus replication in human cells using cryo-electron tomography.