Targeted editing of CCL5 with CRISPR-Cas9 nanoparticles enhances breast cancer immunotherapy.

Breast cancer remains one of the leading causes of cancer-related mortality among women worldwide. Immunotherapy, a promising therapeutic approach, often faces challenges due to the immunosuppressive tumor microenvironment. This study explores the innovative use of CRISPR-Cas9 technology in conjunction with FCPCV nanoparticles to target and edit the C-C Motif Chemokine Ligand 5 (CCL5) gene, aiming to improve the efficacy of breast cancer immunotherapy.

Morphological Characteristics and Extracellular Matrix Abnormalities in Astrocytes Derived From iPSCs of Children With Alexander Disease.

Aims: Alexander disease (AxD) is a leukodystrophy caused by mutations in the astrocytic filament gene GFAP. There are currently no effective treatments for AxD. Previous studies have rarely established AxD models with the patient's original GFAP mutations.

Efficient ternary organic solar cells with suppressed nonradiative recombination via B‒N based polymer donor.

Organic solar cells (OSCs) have developed rapidly in recent years. However, the energy loss ( ) remains a major obstacle to further improving the photovoltaic performance. To address this issue, a ternary strategy has been employed to precisely tune the and boost the efficiency of OSCs.

ubiquitin ligase regulates dichotomous spermatogenesis in .

Spermatogenesis in Lepidoptera holds significant importance due to its unique process of dichotomous spermatogenesis, yielding eupyrene and apyrene spermatozoa through a complex molecular mechanism. While E3 ubiquitin ligases are known to play vital roles in spermatogenesis across various processes, their functions in dichotomous spermatogenesis remain less known. We utilized the RNAi, biochemical and microscopic procedures to unravel the function of in dichotomous spermatogenesis of adult .

Effects of pulsatile flow on postoperative recovery in adult cardiac surgery with cardiopulmonary bypass: A systematic review and meta-analysis of randomized controlled trials.

Background: The role of pulsatile versus non-pulsatile flow during cardiopulmonary bypass (CPB) is still in debate. This systematic review aimed to comprehensively assess the impact of pulsatile versus non-pulsatile flow on patients' recovery.

Methods: We searched MEDLINE, EMBASE, and Cochrane Library databases for randomized controlled trials comparing pulsatile and non-pulsatile flow in cardiac surgeries with CPB.