Stability of insulin lispro in insulin infusion systems.

Objective: To test stability of insulin lispro in two insulin infusion systems over 48 h.

Research Design And Methods: We used reverse-phase and size-exclusion high-performance liquid chromatography (HPLC) to determine the purity, potency, and degree of polymerization of U100 insulin lispro (Humalog) after 24- and 48-h pump cycles conducted at 37 degrees C in five Disetronic H-TRON V100 and five MiniMed 504 pumps. Pumps were set to deliver a basal rate of 0.

Carotid microendarterectomy.

The illumination and magnification provided by the operating microscope allow for the accurate and complete removal of atherosclerotic plaque from the carotid arterial wall, for precise arterial repair at the distal end of the internal carotid endarterectomy, and for a fine, nonstenosing arteriotomy closure. A high dissection of the internal carotid artery, allowing arterial exposure above the plaque, is particularly helpful in the performance of carotid microendarterectomy. The technique of carotid microendarterectomy has been used in a consecutive series of 60 patients with symptomatic carotid stenosis, all but 5 of whom had carotid stenoses of 70% or more.

Gauze-induced granuloma ("gauzoma"): an uncommon complication of gauze reinforcement of berry aneurysms.

Incompletely and even adequately clipped berry aneurysms are often reinforced with finely shredded gauze. In seven female patients this practice led to a series of events including headache, pyrexia, seizures, cranial nerve deficits, endocrinopathy, cerebrospinal fluid pleocytosis, and an enhancing mass demonstrated by computerized tomography at the aneurysm site. One patient with blindness, hydrocephalus, and panhypopituitarism died and was examined at autopsy.

In vivo bicarbonate deficiency and insulin dissolution.

Exogenous insulin exists primarily as the monomer in human plasma. However, in U100 regular insulin formulations, the concentrations of zinc and peptide are such that the insulin hexamer predominates. The biologic result is further disassociation to the monomer after subcutaneous or i.

A technique for rapid non-suture vascular anastomosis.

In an attempt to overcome some of the difficulties inherent in vascular anastomosis using standard suture methods, we have developed a technique that allows for rapid non-suture anastomosis of blood vessels. The technique has been used in over 400 anastomoses, including interposition vein grafts in rat, rabbit and dog vessels ranging in size from 0.7 mm to 3 mm in diameter.