Publications by authors named "W L Hutton"

Here, we provide the genome sequence of a isolated from a screen of an environmental bacterial isolate library for resistance to the plant flavonoid berberine. We detected the colistin resistance gene , located on an IncFII(pECLA) plasmid.

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Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems. Our ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described here, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.

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A reusable water bottle was swabbed as part of the citizen science project "Swab and Send," and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole-genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic-resistant genes, bla , and a variant of fosA, which is likely to explain the observed resistance to ampicillin, amoxicillin, and fosfomycin.

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Background: To compare the stability of fixed- versus variable-angle locking constructs for the comminuted distal humerus fracture (AO/OTA 13-A3).

Methods: Eight pairs of complete humeri harvested from eight fresh frozen cadavers were used for the study. We fixed the intact humeri using 2.

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Objective: Cervical total disk replacement (TDR) has emerged as a motion-preserving alternative to anterior cervical diskectomy fusion (ACDF). Biomechanical studies have demonstrated that the TDR preserves motion at the diseased segment and minimizes motion and stress at adjacent segments compared with fusion. There has been growing interest in performing a TDR adjacent to a cervical fusion.

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