Optimal conditions for the assay of lipoamide dehydrogenase in homogenized human platelets.

Widely different method have been used to assay lipoamide dehydrogenase in tissues from patients with neurological diseases. We have re-examined conditions of assay in homogenized human platelets in the light of results of optimal and inhibitory conditions others have found for the purified pig and rat liver enzymes. Optimal conditions in homogenized platelets for the forward, physiological direction were pH 8.

Serum linoleic acid in multiple sclerosis.

Total serum fatty acids were analyzed in 30 patients with multiple sclerosis and in 33 controls. No significant decrease in linoleic acid was found in the patients with multiple sclerosis. A disturbance in linoleic acid metabolism is not inevitably associated with this disease.

The effect of the environmental temperature on the fatty acid composition and on thein vivo incorporation of 1-(14)C-acetate in goldfish (Carassius auratus L.).

Two-year-old goldfish were adapted to 10C and 35C environmental temperatures during a three-week period, and the fatty acids from triglycerides and certain phospholipids were analyzed by gas-liquid chromatography. Over-all unsaturation of the major fatty acids increased with lower temperature in all lipids which were examined although fish maintained at 10C actually had less polyenoic acid in their tissues than did those maintained at 35C.Fish acclimated to 10C and 30C were injected with 1-(14)C-acetate, and the activities of the isolated fatty acids were counted.