Optimization of three-dimensional printing parameters for orthodontic applications.

Objectives: To determine the impact of build orientation, increased layer thickness, and dental crowding on the trueness of three-dimensional (3D)-printed models, and to evaluate how these parameters affect the fit of thermoformed appliances.

Materials And Methods: Ninety-six dental models were printed horizontally and vertically on the building platform using different 3D-printing technologies: (1) a stereolithography (SLA) printer with layer thicknesses of 160 μm and 300 μm and (2) a digital light processing (DLP) printer with layer thicknesses of 100 μm and 200 μm. Each printed model was digitalized and superimposed on the corresponding source file using 3D rendering software, and deviations were quantified by the root mean square values.

Receptor activator of nuclear factor-kappa B is enriched in CD9-positive extracellular vesicles released by osteoclasts.

Aim: Receptor activator of nuclear factor-kappa B (RANK)-containing extracellular vesicles (EVs) bind RANK-Ligand (RANKL) on osteoblasts, and thereby simultaneously inhibit bone resorption and promote bone formation. Because of this, they are attractive candidates for therapeutic bone anabolic agents. Previously, RANK was detected in 1 in every 36 EVs from osteoclasts by immunogold electron microscopy.

Metabolomic signatures distinguish extracellular vesicles from osteoclasts and odontoclasts.

Aims: Pathological dental root resorption and alveolar bone loss are often detected only after irreversible damage. Biomarkers in the gingival crevicular fluid or saliva could provide a means for early detection; however, such biomarkers have proven elusive. We hypothesize that a multiomic approach might yield reliable diagnostic signatures for root resorption and alveolar bone loss.

The effects of corticotomy and piezocision in orthodontic canine retraction: a randomized controlled clinical trial.

Background: The aims of this study were to evaluate the efficacy of alveolar corticotomy (AC) and piezocision (PZ) in accelerating maxillary canine retraction, and their effects on multiple bone remodeling expression in gingival crevicular fluid (GCF). A split-mouth, randomized controlled clinical trial was performed at the Department of Orthodontics of Pontifical Catholic University of Minas Gerais, Brazil. Eligibility criteria included orthodontic need for first maxillary premolars extractions, followed by canine retraction.

RANKL and RANK in extracellular vesicles: surprising new players in bone remodeling.

Receptor activator of nuclear factor kappa B-ligand (RANKL), its receptor RANK, and osteoprotegerin which binds RANKL and acts as a soluble decoy receptor, are essential controllers of bone remodeling. They also play important roles in establishing immune tolerance and in the development of the lymphatic system and mammary glands. In bone, RANKL stimulates osteoclast formation by binding RANK on osteoclast precursors and osteoclasts.