[Revitalizing donor livers after cardiovascular arrest with venous oxygen persufflation].

The experimental study investigates the resuscitation of livers procured from non heart beating donors by venous systemic oxygen persufflation in a pig transplantation model. In group 1 livers were harvested from donor animals after 60 min of potassium induced cardiac arrest. Livers were then flushed and preserved in 4 degrees C cold University of Wisconsin solution for 4 hrs.

[Differential effect of preservative solutions (UW vs HTK) on mitochondrial redux status and energy metabolism during liver ischemia with oxygen persufflation].

Venous-systemic oxygen persufflation (VSOP) was performed in rat livers stored at 4 degrees C in either UW or HTK preservation solution. Since tissue anoxia is associated with a transformation of cellular NAD+ to NADH and the latter fluoresces upon UV-epiillumination, homogeneity and intensity of liver oxygenation could be analysed by intravital microscopic detection of NADH fluorescence. VSOP resulted in a significant decrease of the NADH signal, documenting effective tissue oxygenation in both UW and HTK.

[Gaseous oxygen for protection and conditioning of organs during ischemia].

During organ ischemia, oxygen (O2) is the first "substrate", which is depleted. However, during ischemic storage in hypothermia (0-4 degrees C), a sufficient oxygenation is attainable by means of gaseous O2. The results of organ preservation were (mostly) better than those obtained with other methods at the respective times.