Purine nucleotides and AMP deamination during maximal and endurance swimming exercise in heart and skeletal muscle of rats.

The purine nucleotides, phosphocreatine (PCr), ammonia, and lactic acid were investigated in skeletal muscles of rats with prominent type I, type IIa, type IIb fibers, and the heart after exhaustive and endurance swimming tests. ATP, ADP, AMP, IMP, and PCr were determined by HPLC with UV detection in controls after maximal and endurance training for 6 weeks with or without a respective final test and also after final exhaustive or endurance test without preceding training. The swimming time in these tests was longer with than without training.

Blood ammonia determination in a specific field test as a method supporting talent selection in runners.

Twenty-six male runners aged 14-16 years not yet specifically trained for sprint or middle-distance running performed a field test with distances of 25 m, 75 m, and 1000 m. Before the field test they were designated as sprinters (n = 19) and middle-distance runners (n = 7) according to their self-assessment and the estimation of the coaches. Blood ammonia and lactate concentrations were measured before and after the runs, and heart rates as well as the perceived exertion were evaluated.

Influence of peripheral arterial occlusive disease on muscular metabolism. Part 1: Changes in lactate, ammonia, and hypoxanthine concentration in femoral blood.

The concentrations of lactate, ammonia and hypoxanthine were determined in blood from the femoral artery, femoral vein and cubital vein under resting conditions in 23 patients with stage II, 10 patients and 20 diabetics with stage IV peripheral arterial occlusive disease (PAOD) and in 19 healthy subjects. The metabolite concentrations were also measured immediately and 20 min after calf exercise in the patients with stage II PAOD and in the controls. At rest, there was a negative arteriovenous difference in femoral lactate level and a positive arteriovenous difference in the ammonia level in all groups.

[Comparison of 3 latest-generation glucose micro-reflectometers].

Three glucose reflectance meters (Reflolux II = Accu-Chek II, Glucometer II and Hypocount GA) were tested for precision and accuracy when used by medical personnel of a diabetic outpatient department and for self-monitoring at home. In addition, the visual readability of the appropriate reagent strip was checked. All three systems were sufficiently valid for reflectometric reading, while visual evaluation showed a higher deviation.