The benefit of culture-independent methods to detect bacteria and fungi in re-infected root filled teeth: a pilot study.

Aim: To identify dominant microorganisms in root filled teeth with apical periodontitis by Pan-PCRs in comparison with a culture-dependent approach, focusing on fungal species profiling.

Methodology: The root filling material (gutta-percha) removed from 42 teeth with periapical radiolucencies undergoing root canal retreatments was analysed by molecular genetics techniques. Real-Time Pan-PCRs were conducted for the diagnosis of predominant bacteria (targeting 16S rDNA) and fungi (targeting ITS1-2 region).

Notch Signaling Mediates Differentiation in Barrett's Esophagus and Promotes Progression to Adenocarcinoma.

Background & Aims: Studies are needed to determine the mechanism by which Barrett's esophagus (BE) progresses to esophageal adenocarcinoma (EAC). Notch signaling maintains stem cells in the gastrointestinal tract and is dysregulated during carcinogenesis. We explored the relationship between Notch signaling and goblet cell maturation, a feature of BE, during EAC pathogenesis.

Smear layer removal efficiency using apple vinegar: An in vitro scanning electron microscope study.

Purpose: To evaluate and compare the smear layer removal efficacy using two different concentrations of apple vinegar.

Methods: 48 single-rooted human teeth with conical roots and canals were randomly divided into four groups and prepared by using a nickel-titanium rotary system (Flexmaster). The final irrigation regimens used were: Group A (negative control group) in which distilled water only was used: Group B (positive control group) in which 2.

promotes colorectal cancer by inducing Wnt/β-catenin modulator Annexin A1.

, a Gram-negative oral anaerobe, is a significant contributor to colorectal cancer. Using an cancer progression model, we discover that stimulates the growth of colorectal cancer cells without affecting the pre-cancerous adenoma cells. Annexin A1, a previously unrecognized modulator of Wnt/β-catenin signaling, is a key component through which exerts its stimulatory effect.