Phenotypic and Genotypic Analysis of Antimicrobial Resistance in Isolated from Pigs with Enzootic Pneumonia in Australia.

, an important cause of enzootic pneumonia in pigs in many countries, has recently been shown to exhibit reduced susceptibility to several antimicrobial classes. In the present study, a total of 185 pig lung tissue samples were collected from abattoirs in Australia, from which 21 isolates of were obtained. The antimicrobial resistance profile of the isolates was determined for 12 antimicrobials using minimum inhibitory concentration (MIC) testing, and a subset ( = 14) underwent whole-genome sequence analysis.

How Malaysian Adolescents Navigate Sexual Health Information on the Internet.

Significant sociocultural barriers to sexual and reproductive health (SRH) information influence what is acceptable to discuss and learn within the Malaysian community. To address gaps in their knowledge, adolescents routinely turn to the Internet. This study identifies the ways they navigate SRH information found online.

Unravelling Antimicrobial Resistance in : Genetic Mechanisms and Future Directions.

Antimicrobial resistance (AMR) in , the causative agent of Enzootic Pneumonia in swine, poses a significant challenge to the swine industry. This review focuses on the genetic foundations of AMR in , highlighting the complexity of resistance mechanisms, including mutations, horizontal gene transfer, and adaptive evolutionary processes. Techniques such as Whole Genome Sequencing (WGS) and multiple-locus variable number tandem repeats analysis (MLVA) have provided insights into the genetic diversity and resistance mechanisms of .

DNA methylation analysis to differentiate reference, breed, and parent-of-origin effects in the bovine pangenome era.

Background: Most DNA methylation studies have used a single reference genome with little attention paid to the bias introduced due to the reference chosen. Reference genome artifacts and genetic variation, including single nucleotide polymorphisms (SNPs) and structural variants (SVs), can lead to differences in methylation sites (CpGs) between individuals of the same species. We analyzed whole-genome bisulfite sequencing data from the fetal liver of Angus (Bos taurus taurus), Brahman (Bos taurus indicus), and reciprocally crossed samples.