Protection against feline infectious peritonitis by intranasal inoculation of a temperature-sensitive FIPV vaccine.

Cats vaccinated intranasally (i.n.) with a temperature sensitive feline infectious peritonitis virus (ts-FIPV) vaccine were protected against an FIP-inducing challenge.

Evaluation of immunosuppressive effect and efficacy of an improved-potency feline leukaemia vaccine.

An inactivated feline leukaemia vaccine was tested to determine if process improvements would permit it to be effectively used in a two-dose primary regimen versus the three-dose regimen required for a previous vaccine. Twenty-five cats were vaccinated with two subcutaneous doses given 3 weeks apart. Vaccinates and controls were artificially immunosuppressed to enhance susceptibility to challenge, and inoculated with virulent feline leukaemia virus (FeLV) 2 weeks after the second dose.

Characterization of an attenuated temperature sensitive feline infectious peritonitis vaccine virus.

Intranasal administration of a ts-FIPV vaccine protected cats against two rigorous challenges of immunity. Investigations showed that ts-FIP viral RNA synthesis was normal at 39 degrees C and structural proteins were synthesized, but not expressed at the cell surface. Lack of surface expression combined with decreased virus titer indicate that, although structural viral proteins were initially synthesized, they were not packaged into intact virions at the nonpermissive temperature.

Identification of pseudorabies virus-exposed swine with a gI glycoprotein enzyme-linked immunosorbent assay.

A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted virus. The gI ELISA was evaluated by comparing it with the virus neutralization test and with a standard ELISA which does not distinguish between vaccinated and naturally infected animals.

Efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle.

Efficacy and safety of components of an IM-administered vaccine for prevention of infectious bovine rhinotracheitis virus (IBRV), parainfluenza type-3 (PI-3) virus, bovine viral diarrhea virus (BVDV), and respiratory syncytial virus (RSV) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. Challenge of immunity tests were conducted in calves for IBRV, PI-3 virus, or BVDV vaccinal components. All inoculated calves developed serum-neutralizing antibodies and had substantially greater protection (as measured by clinical rating systems) than did controls after challenge exposure to virulent strains of IBRV, PI-3 virus, BVDV, or RSV.