Differentiation of Lactobacillus isolates from infant faeces by SDS-PAGE and rRNA-targeted oligonucleotide probes.

Isolates of lactobacilli from infant faeces phenotypically characterized as Lactobacillus paracasei subsp. paracasei (six strains), Lact. rhamnosus (six strains), Lact.

Expression of Lactobacillus casei ATCC 393 beta-galactosidase encoded by plasmid pLZ15 in Lactococcus lactis CNRZ 1123.

Lactococcus lactis subsp. lactis CNRZ 1123, a Lac- derivative of CNRZ 1122 was transformed by electroporation with the Lactobacillus casei ATCC 393 plasmid pLZ15, which bears a beta-galactosidase gene. The transformants expressed a constitutive beta-galactosidase activity at a higher level than in Lact.

Cloning and expression of the lysostaphin gene in Bacillus subtilis and Lactobacillus casei.

The lysostaphin structural gene was cloned in Bacillus subtilis DSM402 and in Lactobacillus casei 102S. The gene was expressed in both organisms and active lysostaphin was released into the medium. Lysostaphin produced by these organisms induced lysis of growing and heat inactivated staphylococci.

Expression of the lipase gene from Staphylococcus hyicus in Lactobacillus curvatus Lc2-c.

An electroporation based transformation system was developed for Lactobacillus curvatus Lc2-c, a plasmid cured potential raw sausage starter. It was transformed with plasmid pLipPS1, a staphylococcal vector plasmid containing the lipase gene from Staphylococcus hyicus. Transformants exhibited high lipase activity, whereas the wild type strain was lipase negative.