Publications by authors named "W Eskild"
Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis.
PLoS One
November 2023
Article Synopsis
- - Liver sinusoidal endothelial cells (LSECs) play a crucial role in clearing waste from blood, but their function is affected in liver disease, particularly in Glmpgt/gt mice, which show chronic liver injury and fibrosis.
- - A study using FITC-labelled serum albumin demonstrated that LSECs in Glmpgt/gt mice have significantly reduced capacity for scavenging compared to wild-type mice, even though certain scavenger receptor expressions remained similar.
- - Despite the presence of increased collagen and liver damage, LSECs in Glmpgt/gt mice maintained well-formed fenestrae, though with a higher frequency of larger openings, indicating alterations in structure but not a complete loss of function.
Multifactorial metabolic diseases, such as non-alcoholic fatty liver disease, are a major burden to modern societies, and frequently present with no clearly defined molecular biomarkers. Herein we used system medicine approaches to decipher signatures of liver fibrosis in mouse models with malfunction in genes from unrelated biological pathways: cholesterol synthesis-, notch signaling-, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling-, and unknown lysosomal pathway-. Enrichment analyses of Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome and TRANScription FACtor (TRANSFAC) databases complemented with genome-scale metabolic modeling revealed fibrotic signatures highly similar to liver pathologies in humans.
View Article and Find Full Text PDFLysosomes are major sites for intracellular, acidic hydrolase-mediated proteolysis and cellular degradation. The export of low-molecular-weight catabolic end-products is facilitated by polytopic transmembrane proteins mediating secondary active or passive transport. A number of these lysosomal transporters, however, remain enigmatic.
View Article and Find Full Text PDFBackground And Aims: Vascular endothelial growth factor (VEGF) receptors (VEGFR1 and VEGFR2) bind VEGF-A with high affinity. This study sought to determine the relative contributions of these two receptors to receptor-mediated endocytosis of VEGF-A and to clarify their endocytic itineraries in rat liver sinusoidal endothelial cells (LSECs).
Methods: Isolated LSECs and radiolabeled VEGF-A were used to examine surface binding and receptor-mediated endocytosis.
Age-dependent development of liver fibrosis in Glmp (gt/gt) mice.
Fibrogenesis Tissue Repair
May 2016
Background: Mice lacking glycosylated lysosomal membrane protein (Glmp (gt/gt) mice) have liver fibrosis as the predominant phenotype due to chronic liver injury. The Glmp (gt/gt) mice grow and reproduce at the same rate as their wild-type siblings. Life expectancy is around 18 months.
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