The use of epostane, a 3(beta)-hydroxysteroid dehydrogenase delta 4-5 isomerase enzyme inhibitor, in oil suspension as a mismating agent in the dog.

Administration of epostane as a single subcutaneous injection in sesame oil prevented or terminated pregnancy in bred beagle bitches (P = 0.002). Doses of 15 and 20 mg/kg prevented or terminated pregnancy in all of 14 bred beagle bitches studied but demonstrated a high rate of serious abscess formation at the injection site.

Drug disposition and biotransformation in the developing beagle dog.

The ability of developing male and female beagle pups to biotransform and eliminate drugs was studied by administering single intravenous doses of acetaminophen (50 mg/kg body wt), phenobarbital (15 mg/kg body wt), or phenytoin (15 mg/kg body wt) to the same groups of dogs (n = 6-8/drug) at 4, 10, 20, 40, and 60 days of age. At suitable intervals after treatment, small (1.0 ml) blood samples were obtained via the jugular vein and centrifuged and the plasma was recovered and stored at -20 degrees C to await analysis.