Publications by authors named "W E Gaines"

We evaluated ecosystem conditions known to influence the viability of a strictly arboreal species (the red tree vole, ) endemic and historically distributed in the forests across the Coast Range, Cascades, and Klamath Mountains ecoregions in the Western United States of America. We found widespread reductions in ecosystem conditions needed to support the long-term viability of the red tree vole. This was particularly evident in the Coast Range where the weighted watershed index (WWI) was 26% of its historical value, and the current probability of maintaining viability departed the most from historical viability probabilities in ecoregions that were evaluated.

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Anthropogenic and natural habitat fragmentation inhibit movement of animals through landscapes. An important challenge for connectivity conservation is determining which conditions facilitate or limit movements, so that these areas can be prioritized for protection or restoration. We examine Canada lynx Lynx canadensis habitat connectivity in the fragmented North Cascade Mountains of Washington, as an example of a highly mobile species that is specialized both on prey and in habitat needs.

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Article Synopsis
  • During meiosis, double strand breaks (DSBs) are repaired preferentially between homologous chromosomes, relying on proteins Rad51 and Dmc1 for interhomolog bias and meiotic recombination.
  • In dmc1Δ mutants, Rad51 is inhibited, leading to prophase arrest due to unresolved DSBs, with this inhibition dependent on the kinase Mek1 acting through two mechanisms that prevent Rad51’s accessory factor Rad54 from forming complexes.
  • This study reveals that Mek1 phosphorylates Hed1, strengthening its ability to suppress Rad51 activity, thus ensuring proper chromosome crossover and stability during meiosis.
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The high tensile strength and biocompatibility of spider dragline silk makes it a desirable material in many engineering and tissue regeneration applications. Here, we present the feasibility to produce recombinant proteins in transgenic tobacco Nicotiana tabacum with sequences representing spider silk protein building blocks . Recombinant mini-spidroins contain native N- and C-terminal domains of major ampullate spidroin 1 (rMaSp1) or rMaSp2 flanking an abbreviated number (8, 16 or 32) of consensus repeat domains.

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