MicroRNA expression profiling of megakaryocytes in primary myelofibrosis and essential thrombocythemia.

In primary myelofibrosis (PMF) and essential thrombocythemia (ET) the megakaryocytic lineage characteristically shows aberrant proliferation and maturation in which the regulatory microRNA (miR) system might be involved. Laser-microdissected PMF and ET megakaryocytes were analysed with real-time polymerase chain reaction (PCR) low density arrays comprising 365 microRNAs. The highest megakaryocytic expression levels were observed for miR-223, which is known to be expressed also in granulopoiesis.

Megakaryocytic expression of miRNA 10a, 17-5p, 20a and 126 in Philadelphia chromosome-negative myeloproliferative neoplasm.

Micro RNA (miRNA) are small non-coding RNA molecules which have a post-transcriptional inhibitory regulation function, e.g. in megakaryopoiesis.

Rifabutin in the treatment of cavitary lung disease due to Mycobacterium gordonae.

We have described a case of progressive pulmonary disease caused by M gordonae that failed to show any improvement in disease status after 9 months of intensive therapy with a conventional four-drug regimen. When rifabutin became available and was included in the regimen, the disease was cured. Although considered a saprophyte of the respiratory tract, M gordonae can cause clinically significant disease in rare instances.