Accurate and fast segmentation of filaments and membranes in micrographs and tomograms with TARDIS.

It is now possible to generate large volumes of high-quality images of biomolecules at near-atomic resolution and in near-native states using cryogenic electron microscopy/electron tomography (Cryo-EM/ET). However, the precise annotation of structures like filaments and membranes remains a major barrier towards applying these methods in high-throughput. To address this, we present TARDIS (ransformer-bsed apid imensionless nstance egmentation), a machine-learning framework for fast and accurate annotation of micrographs and tomograms.

HEMATOLOGY AND BIOCHEMICAL REFERENCE INTERVALS AND SEROPREVALENCE OF HEMORRHAGIC DISEASES FOR FREE-RANGING MULE DEER () IN WEST TEXAS.

Wildlife species are routinely captured for translocation, general health monitoring, and research-based pursuits to guide wildlife management. Mule deer () were captured for various research projects and management actions in the Trans-Pecos and Panhandle regions of Texas from 2015 to 2019. The objective of this study was to develop hematologic and biochemical parameters for free-ranging mule deer in Texas and to develop a health monitoring system for current and future mule deer population management.

HEMATOLOGY AND BIOCHEMICAL REFERENCE INTERVALS FOR FREE-RANGING PRONGHORN () IN WEST TEXAS.

Pronghorn () are considered a keystone species of North American grasslands and an important economic source for many landowners in Texas. Pronghorn restoration projects routinely capture and translocate individuals from surplus populations to restoration areas. The objective of this study was to generate normal hematological and biochemical reference intervals (RI) for free-ranging pronghorn populations in Texas as a health monitoring tool for pronghorn restoration efforts.

Inactivation of Salmonella Enteritidis PT30 on black peppercorns in thermal treatments with controlled relative humidities.

Traditional method utilizes steam to pasteurize low-moisture ingredients like black peppercorns and almonds. Exposure to steam results in direct condensation on the product, unfavorable for a broader range of food ingredients such as dried herbs, fruits, and ground materials. Recent studies on the thermal inactivation of Salmonella in low-moisture foods suggest that the relative humidity in treatment chambers is an important factor, besides temperature, that determines the death rate of bacteria.

Three-dimensional structure of kinetochore-fibers in human mitotic spindles.

During cell division, kinetochore microtubules (KMTs) provide a physical linkage between the chromosomes and the rest of the spindle. KMTs in mammalian cells are organized into bundles, so-called kinetochore-fibers (k-fibers), but the ultrastructure of these fibers is currently not well characterized. Here, we show by large-scale electron tomography that each k-fiber in HeLa cells in metaphase is composed of approximately nine KMTs, only half of which reach the spindle pole.