Increased Salt and Drought Tolerance by D-Ononitol Production in Transgenic Nicotiana tabacum L.

A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 [mu]mol g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. One day after rewatering drought-stressed plants, I5A photosynthesis had recovered 75% versus 57% recovery with cultivar SR1 plants.

Sorbitol-6-phosphate dehydrogenase expression in transgenic tobacco. High amounts of sorbitol lead to necrotic lesions.

We analyzed transgenic tobacco (Nicotiana tabacum L.) expressing Stpd1, a cDNA encoding sorbitol-6-phosphate dehydrogenase from apple, under the control of a cauliflower mosaic virus 35S promoter. In 125 independent transformants variable amounts of sorbitol ranging from 0.

Growth and development of Mesembryanthemum crystallinum (Aizoaceae).

This review describes the life cycle of Mesembryantheum crystallinum L. (the common ice plant, Aizoaceae, Caryophyllales), a halophyte with a developmentally programmed switch from C photosynthesis to Crassulacean acid metabolism (CAM) which is accelerated by salinity and drought. Since there has been controversy regarding the interplay between genes and environmental stimuli during the development of M.