An integrated microflow cytometry platform with artificial intelligence capabilities for point-of-care cellular phenotype analysis.

The EZ DEVICE is an integrated fluorescence microflow cytometer designed for automated cell phenotyping and enumeration using artificial intelligence (AI). The platform consists of a laser diode, optical filter, objective lens, CMOS image sensor, and microfluidic chip, enabling automated sample pretreatment, labeling, and detection within a single compact unit. AI algorithms segment and identify objects in images captured by the CMOS sensor at 532 and 586 nm emission wavelengths.

Long-Term Follow-Up of Surgical Outcomes and Oncological Results of Nipple-Sparing Mastectomy with Immediate Reconstruction Through a Single Axillary Incision with Different Approach Methods.

Background: We investigated the perioperative outcome and oncologic safety of performing nipple-sparing mastectomy (NSM) through a single axillary incision (NSM-SAI) compared with performing NSM through a conventional incision (NSM-C).

Methods: We retrospectively reviewed 725 patients who underwent NSM for breast cancer between January 2010 and December 2023; 333 patients who underwent NSM with immediate reconstruction (IR) were enrolled. Surgical outcomes and oncologic outcomes of NSM-C (n = 184), NSM performed through SAI with a freehand approach (NSM-SAI-F; n = 92), and with an endoscopic approach (NSM-SAI-E; n = 57) were demonstrated.

Protogenin facilitates trunk-to-tail HOX code transition via modulating GDF11/SMAD2 signaling in mammalian embryos.

During embryogenesis, vertebral axial patterning is intricately regulated by multiple signaling networks. This study elucidates the role of protogenin (Prtg), an immunoglobulin superfamily member, in vertebral patterning control. Prtg knockout (Prtg) mice manifest anterior homeotic transformations in their vertebral columns and significant alterations in homeobox (Hox) gene expression.

Associations of care continuity and care coordination with the overuse of healthcare services: a nationwide population-based study.

Background: Care continuity and care coordination have received increased attention as important ways of decreasing overuse/low-value care. Prior research has verified an association between care continuity and overuse or an association between care coordination and overuse. However, little is known about the relative influences of care continuity and care coordination on overuse.

Exploring the potential of flour, starch concentrate, and protein concentrate from Richlea lentils in the development of lentil based extruded puffs.

Lentil puffs were developed from a mixed design of varying weight fractions of lentil flour (x1), lentil starch concentrate (x2), and lentil protein concentrate (x3) using a twin-screw pilot scale extruder at a dry feed rate of 20 kg/h (d.b.), a water feed rate of 2 kg/h, and an extruder screw speed of 350 rpm.