Publications by authors named "W Brack"

The increasing number of contaminants released into the environment necessitates innovative strategies for their detection and identification, particularly in complex environmental matrices like hospital wastewater. Hospital effluents contain both natural and synthetic hormones that might significantly contribute to endocrine disruption in aquatic ecosystems. In this study, HT-EDA has been implemented to identify the main effect-drivers (testosterone, androsterone and norgestrel) from hospital effluent using microplate fractionation, the AR-CALUX bioassay and an efficient data processing workflow.

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Steroid hormones are significant contributors to endocrine disruption, affecting the hormonal functions of both humans and aquatic organisms. However, data on their occurrence and risks in fresh water systems particularly in low- and middle-income countries, is scarce. In this regard, a comprehensive investigation of 58 steroid hormones in rivers and wastewater treatment plants (WWTPs) was conducted in western Kenya.

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Indoor dust contains various endocrine-disrupting contaminants, yet the effect drivers of observed glucocorticoid activity are completely unknown. This study conducted an effect-directed analysis using orthogonal fractionation to identify effect drivers of glucocorticoid activity in indoor dust. After the detection of bioactivity using a human cell line stably transfected with a reporter gene, the sample underwent parallel HPLC fractionations with octadecyl, pentafluorophenyl, and aminopropyl columns to obtain orthogonal fractions.

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Increasing chemical pollution calls for a closer look at ecologically highly relevant host-parasite interactions to understand the persistence of organisms and populations in a polluted environment. The impact of chemical exposure within the host-parasite interactions - particularly the distinctive bioaccumulation behavior of organic micropollutants - can substantially influence the persistence of a species. This significance has been emphasized by previous research showing a higher tolerance of Gammarus roeselii (Amphipoda, Crustacea) infected with acanthocephalans during acute exposure to a pyrethroid.

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Bacterial communities respond to environmental conditions with diverse structural and functional changes depending on their compartment (water, biofilm or sediment), type of environmental stress, and type of pollution to which they are exposed. In this study, we combined amplicon sequencing of bacterial 16S rRNA genes from water, biofilm, and sediment samples collected in the anthropogenically impacted River Aconcagua basin (Central Chile, South America), in order to evaluate whether micropollutants alter bacterial community structure and functioning based on the type and degree of chemical pollution. Furthermore, we evaluated the potential of bacterial communities from differently polluted sites to degrade contaminants.

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