Oral flora meningoencephalitis diagnosis by next-generation DNA sequencing.

Introduction: Standard culture methods may fail to detect the causative agents of bacterial infection for various reasons including specimen collection after antibiotic administration, or when standard techniques or environmental conditions are not appropriate for growth of the microorganisms. Conventional 16S rRNA gene sequencing is sometimes a useful alternative technique for identification of bacteria, but is confounded by polymicrobial infection. We present a case of a patient who developed a serious neurological infection for which causative oral flora organisms were observed by microscopy, failed to culture but were identified by next-generation DNA sequencing.

Attitudes affecting the potential use of human papillomavirus vaccination: a survey of health promotion students in Mexico City.

Our aim was to explore the knowledge and attitudes of Mexican college students towards human papillomavirus (HPV) vaccination. We conducted a written questionnaire survey of a group of male and female undergraduate students in Health Promotion at the Autonomous University of Mexico City. A total of 163 subjects (40 males and 123 females, median age 24 years) took part.

Human papillomavirus vaccine-recent results and future developments.

A subset of human papillomaviruses that infect the anogenital tract and the upper aero-digestive tract is the cause of a number of benign and malignant tumors in these locations, including cervical cancer. Since the past year, a vaccine directed at human papillomaviruses types 6 and 11, the main cause of genital warts, and types 16 and 18, the main cause of cervical cancer, has been on the international market. Another vaccine directed at types 16 and 18 alone is soon expected to be widely available.