Primitive processes, metaphor, and recognition in the treatment of traumatic loss.

This paper explores how traumatic loss and emotional abuse ruptured a patient's symbolic process and shattered her experience of the reality of her self. In treatment, metaphoric investigation of physical processes of expulsion and incorporation led to a transformation of projective identification into the containment she sought. The therapist's ability to metabolize pain, shame, and the risks of incestuous merger re-experienced in the treatment grew out of his recognition of disturbing experiences of his own that she brought to life.

Organ-, inflammation- and cancer specific transcriptional fingerprints of pancreatic and hepatic stellate cells.

Background: Tissue fibrosis is an integral component of chronic inflammatory (liver and pancreas) diseases and pancreatic cancer. Activated pancreatic- (PSC) and hepatic- (HSC) stellate cells play a key role in fibrogenesis. To identify organ- and disease-specific stellate cell transcriptional fingerprints, we employed genome-wide transcriptional analysis of primary human PSC and HSC isolated from patients with chronic inflammation or cancer.

Genome-wide transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines.

With the use of genome-wide cDNA microarrays, we investigated the transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines. In all, 1,098 chip entries were differentially regulated in the two cell lines; of these, 796 entries corresponded to characterized mRNAs. The identified genes are mostly expressed in epithelial tissues and localize on chromosomes 1, 10, and 20.

Next-generation DNA sequencing techniques.

Next-generation high-throughput DNA sequencing techniques are opening fascinating opportunities in the life sciences. Novel fields and applications in biology and medicine are becoming a reality, beyond the genomic sequencing which was original development goal and application. Serving as examples are: personal genomics with detailed analysis of individual genome stretches; precise analysis of RNA transcripts for gene expression, surpassing and replacing in several respects analysis by various microarray platforms, for instance in reliable and precise quantification of transcripts and as a tool for identification and analysis of DNA regions interacting with regulatory proteins in functional regulation of gene expression.

Molecular mechanisms of the antiangiogenic and antitumor effects of mycophenolic acid.

The relative risk for the development of malignancies following solid organ transplantation seems to be decreased in patients treated with the immunosuppressive agent mycophenolic acid (MPA). However, the molecular mechanisms of the antineoplastic effects of MPA are not completely understood. Here, we report that human endothelial cells and fibroblasts are highly sensitive to MPA treatment.