Publications by authors named "W A Parrott"

This strategic plan summarizes the major accomplishments achieved in the last quinquennial by the soybean [Glycine max (L.) Merr.] genetics and genomics research community and outlines key priorities for the next 5 years (2024-2028).

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Screening a transposon-mutagenized soybean population led to the discovery of a recessively inherited chlorotic phenotype. This "y24" phenotype results in smaller stature, weaker stems, and a smaller root system. Genome sequencing identified 15 candidate genes with mutations likely to result in a loss of function.

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Genetic modification of plants fundamentally relies upon customized vector designs. The ever-increasing complexity of transgenic constructs has led to increased adoption of modular cloning systems for their ease of use, cost effectiveness, and rapid prototyping. GreenGate is a modular cloning system catered specifically to designing bespoke, single transcriptional unit vectors for plant transformation-which is also its greatest flaw.

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Article Synopsis
  • - A soybean population with transposon mutations revealed a recessive "vir1" phenotype, characterized by reduced size, weakened structures, and smaller root systems with fewer nodules.
  • - Genome sequencing identified 15 candidate genes, ultimately narrowing down to one crucial mutation that disrupts a gene responsible for splicing, mostly expressed in mesophyll cells and activated by cold stress during germination.
  • - Similar mutations in rice also led to chlorosis under cooler temperatures, and soybean vir1 mutants exhibited worsening symptoms in low temperatures; transgenic restoration in Arabidopsis confirmed the mutation's link to the vir1 phenotype.
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