Proteinase B disruption is required for high level production of human mechano-growth factor in Saccharomyces cerevisiae.

Mechano-growth factor (MGF) is a product of a unique muscle-specific splice variant of the insulin-like growth factor I gene. Potential use of MGF to improve regenerative capability of skeletal muscle as well as to prevent neuronal damage has been widely discussed. Human MGF was expressed in Saccharomyces cerevisiae, but the yield of the recombinant protein was low due to its rapid degradation.

Producing human mechano growth factor (MGF) in Escherichia coli.

MGF is a product of a unique muscle-specific splice variant of IGF1 gene (insulin-like growth factor). Its peculiar feature is a specific E-peptide, a 16 a.a.