Single Interdigital Transducer Approach for Gravimetrical SAW Sensor Applications in Liquid Environments.

Surface acoustic wave (SAW) devices are well known for mass-sensitive sensor applications. In biosensing applications, chemical and biochemically evoked binding processes on surfaces are detected in liquid environments using delay line or resonator sensor configurations, preferably in combination with the appropriate microfluidic devices. All configurations share the common feature of analyzing the transmission characteristic of the propagating SAW.

Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance.

A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush.

Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages.

Surface topography impacts on cell growth and differentiation, but it is not trivial to generate defined surface structures and to assess the relevance of specific topographic parameters. In this study, we have systematically compared in vitro differentiation of mesenchymal stem cells (MSCs) on a variety of groove/ridge structures. Micro- and nano-patterns were generated in polyimide using reactive ion etching or multi beam laser interference, respectively.

Surface enhanced infrared spectroscopy with gold strip gratings.

We investigate surface enhanced infrared absorption (SEIRA) spectroscopy with gold strip gratings made by standard optical lithography. By exciting surface plasmon polaritons on both air-gold and gold-substrate interfaces, the resonance of the 1D gratings is linearly tunable with the grating period. With the field enhancement at the edge of the gold strips, a SEIRA enhancement factor more than 6000 for PMMA molecules is achieved.