Formation of aggregated neutrophil extracellular traps in tissues is determining the efficacy of particulate nanoadjuvants.

Neutrophils are essential for innate immunity, using mechanisms like Neutrophil Extracellular Trap (NET) formation to fight pathogens. Aggregated NETs (aggNETs) help resolve inflammation by cleaving pro-inflammatory cytokines, while scattered NETs can exacerbate inflammation, leading to tissue damage. Co-injection of 10 nm nanodiamonds (ND10) with peptide antigens boosts immune responses, including anti-SARS-CoV-2 immunity, due to transient immune responses induced by aggNETs around ND10 particles.

Endoscopic light-guided choledochoduodenostomy in the treatment of the distal common bile duct obstruction.

Objective: Aim: Experimental justification for creation of bile offtake into the duodenum with minimally invasive methods in cases of obstruction of the distal part of common bile duct and failure of transpapillary interventions and studying the first results of such intervention application.

Patients And Methods: Materials and Methods: The anatomical relationships between the duodenum and the common bile duct in its distal parts starting from its retroduodenal part to the sphincter of Oddi were studied. The possibility of transillumination of the walls of the common bile duct and the duodenum by a light source introduced into the lumen of the common bile duct is determined.

Giant cell arteritis as a cause of extracranial internal carotid artery aneurysm: a case report.

Background: This report presents the management of patient with extracranial internal carotid artery pseudoaneurysm due to giant cell arteritis.

Case Presentation: Left internal carotid artery pseudoaneurysm was diagnosed in a 57-year-old Ukrainian woman, which became a direct indication for surgical treatment involving aneurysm resection and internal carotid artery reimplantation. The used reconstruction technique with oblique cutting of internal carotid artery, aneurysm resection, ellipse-form anastomosis formation, and distal intima fixation prevents the dissection, restenosis, and aneurysm of anastomosis in the long-term postoperative period.

Evaluation of functional interaction masseter and sternocleidomastoid muscles affected by TMJ dysfunction.

Objective: Aim: To evaluate the functional connection and the bioelectrical state of the m.masseter and m. sternocleidomastoid using functional tests before and after treatment.

THE EFFECT OF A MULTIMODAL APPROACH ON THE RESULTS OF TREATMENT IN SURGERY: INTEGRATION OF CHEMOTHERAPY, SURGERY, AND RADIOTHERAPY.

This study investigates the safety and efficacy of a multimodal approach, integrating radiotherapy, chemotherapy, and surgery for the management of cancer patients. This review systematically reviewed English-language literature from digital repositories, namely Web of Science, Scopus, Google Scholar, PubMed, and the Cochrane Library. The search strategy employed a targeted selection of keywords: "chemotherapy," "radiotherapy," "multimodal," and "surgery," encompassing publications published before January 2024.

Neutrophil Activity and Extracellular Matrix Degradation: Drivers of Lung Tissue Destruction in Fatal COVID-19 Cases and Implications for Long COVID.

Pulmonary fibrosis, severe alveolitis, and the inability to restore alveolar epithelial architecture are primary causes of respiratory failure in fatal COVID-19 cases. However, the factors contributing to abnormal fibrosis in critically ill COVID-19 patients remain unclear. This study analyzed the histopathology of lung specimens from eight COVID-19 and six non-COVID-19 postmortems.

COMPARATIVE EVALUATION OF THE TEMPOROMANDIBULAR JOINT STRUCTURES AFFECTED BY TMJ DYSFUNCTION.

Objective: The aim: To investigate the individual anatomical features of the temporomandibular joint structures affected by TMJ disorders and compare them with those of asymptomatic patients.

Patients And Methods: Materials and methods: The sample consisted of 41 patients. The study group included 29 patients.

Evaluating the Influences of Health Expenditure, Energy Consumption, and Environmental Pollution on Life Expectancy in Asia.

This study examines the effects of health expenditure, energy consumption, CO emissions, population size, and income on health outcomes in 46 Asian nations between 1997 and 2019. Cross-sectional dependence (CSD) and slope heterogeneity (SH) tests are utilized due to the close linkages between Asian nations as a result of commerce, tourism, religion, and international agreements. The research uses unit root and cointegration tests of the second generation after validating CSD and SH issues.

Influence of the First Wave of the COVID-19 Pandemic on Cancer Care in a German Comprehensive Cancer Center.

During the first wave of the COVID-19 pandemic in 2020, the German government implemented legal restrictions to avoid the overloading of intensive care units by patients with COVID-19. The influence of these effects on diagnosis and treatment of cancer in Germany is largely unknown. To evaluate the effect of the first wave of the COVID-19 pandemic on tumor board presentations in a high-volume tertiary referral center (the German Comprehensive Cancer Center NCT/UCC Dresden), we compared the number of presentations of gastrointestinal tumors stratified by tumor entity, tumor stage, and treatment intention during the pandemic to the respective data from previous years.

Identification of Factors for the Development of Medical Tourism in the World.

The overall objective of the given paper was to study the relationship of inbound medical tourism destinations with international tourism, economic development of recipient countries, the development of national healthcare systems and the institutional features of their environment, in terms of protection of the rights and freedoms of both business and citizens. In order to achieve this objective, the authors used methods of grouping, as well as correlation and regression analysis. The conducted study revealed that the formation of medical tourism destinations in countries with high social and economic development occurs in a balanced and unidirectional manner; simultaneously, one can see that the countries with "new economic development" form a sufficiently powerful and competitive market for medical tourism.

[ARCHITECTONICS OF THE NIGHT SLEEP BASED ON ITS POLYSOMNOGRAPHIC CHARACTERISTICS IN PATIENTS WITH MULTIPLE SCLEROSIS].

Aim of our study is to establish polysomnographic characteristics of nocturnal sleep in various forms of multiple sclerosis. Fifty eight patients with different clinical course of multiple sclerosis (MS) were included into the study. Disturbances of sleep architectonics were identified: representation of deep stages of NREM sleep, as well as REM sleep were reduced.

CLINICAL SONOGRAPHIC ANALYSIS OF BIOMETRIC INDICATORS OF BUCCAL THICKNESS AND BUCCAL FAT PAD IN PATIENTS WITH DIFFERENT FACIAL TYPES.

Clinical and sonographic study of BFP biometric parameters in patients with different face typology is necessary for proper planning and efficient sampling. Aim of the study was to establish a scientific and practical analysis of clinical sonographic results of the examination of patients with different face types in preparation for reconstruction of the oral tissue defects of the BFP. The study was conducted with 25 patients.

Neutrophils as Main Players of Immune Response Towards Nondegradable Nanoparticles.

Many nano/microparticles (n/µP), to which our body is exposed, have no physiological way of removal. Our immune system sense these "small particulate objects", and tries to decrease their harmfulness. Since oxidation, phagocytosis and other methods of degradation do not work with small, chemically resistant, and hydrophobic nanoparticles (nP).

Clinical laboratory analysis of maximum intercuspation registration results in patients with intact dentitions.

Objective: Introduction: In modern dental prosthetic practice, there often occur inconsistencies of occlusion in oral cavity and on cast dental models, leading to search for a solution to the problem of improving the registration accuracy of occlusal relationships of dentitions. The aim: Clinical laboratory study of bite registration materials selected efficiency.

Patients And Methods: Materials and methods: Examination of 10 patients was conducted.

Reduced Graphene-Oxide-Embedded Polymeric Nanofiber Mats: An "On-Demand" Photothermally Triggered Antibiotic Release Platform.

The steady increase of antimicrobial resistance of different pathogens requires the development of alternative treatment strategies next to the oral delivery of antibiotics. A photothermally activated platform based on reduced graphene oxide (rGO)-embedded polymeric nanofiber mats for on-demand release of antibiotics upon irradiation in the near-infrared is fabricated. Cross-linked hydrophilic nanofibers, obtained by electrospinning a mixture of poly(acrylic acid) (PAA) and rGO, show excellent stability in aqueous media.

Active NET formation in Libman-Sacks endocarditis without antiphospholipid antibodies: A dramatic onset of systemic lupus erythematosus.

Although neutrophil extracellular traps (NETs) have been highlighted in several systemic inflammatory diseases, their clinical correlates and potential pathological role remain obscure. Herein, we describe a dramatic onset of systemic lupus erythematosus (SLE) with clear-cut pathogenic implications for neutrophils and NET formation in a young woman with cardiac (Libman-Sacks endocarditis) and central nervous system (psychosis and seizures) involvement. Despite extensive search, circulating antiphospholipid autoantibodies, a hallmark of Libman-Sacks endocarditis, could not be detected.

Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps.

If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation.

Flexible Nanoholey Patches for Antibiotic-Free Treatments of Skin Infections.

Despite the availability of different antibiotics, bacterial infections are still one of the leading causes of hospitalization and mortality. The clinical failure of antibiotic treatment is due to a general poor antibiotic penetration to bacterial infection sites as well as the development of antibiotic-resistant pathogens. In the case of skin infection, the wound is covered by exudate, making it impermeable to topical antibiotics.

Neutrophil Extracellular Traps Form a Barrier between Necrotic and Viable Areas in Acute Abdominal Inflammation.

Neutrophils form neutrophil extracellular traps (NETs) of decondensed DNA and histones that trap and immobilize particulate matter and microbial pathogens like bacteria. NET aggregates reportedly surround and isolate large objects like monosodium urate crystals, which cannot be sufficiently cleared from tissues. In the setting of acute necrotizing pancreatitis, massive tissue necrosis occurs, which is organized as pancreatic pseudocysts (1).

Early detection of ovarian cancer in samples pre-diagnosis using CA125 and MALDI-MS peaks.

Aim: A nested case-control discovery study was undertaken to test whether information within the serum peptidome can improve on the utility of CA125 for early ovarian cancer detection.

Materials And Methods: High-throughput matrix-assisted laser desorption ionisation mass spectrometry (MALDI-MS) was used to profile 295 serum samples from women pre-dating their ovarian cancer diagnosis and from 585 matched control samples. Classification rules incorporating CA125 and MS peak intensities were tested for discriminating ability.

Machine learning classification with confidence: application of transductive conformal predictors to MRI-based diagnostic and prognostic markers in depression.

There is rapidly accumulating evidence that the application of machine learning classification to neuroimaging measurements may be valuable for the development of diagnostic and prognostic prediction tools in psychiatry. However, current methods do not produce a measure of the reliability of the predictions. Knowing the risk of the error associated with a given prediction is essential for the development of neuroimaging-based clinical tools.

Thermal stability of a Co/Cu giant magnetoresistance (GMR) multilayer system.

Critical limitations exist regarding the thermal stability of multilayer giant magnetoresistance (GMR) sensors due to significant contribution of layer interfaces and grain boundaries to their performance. This paper addresses the stability of, and thermal reaction in Co/Cu magnetoresistive systems. Thin film multilayers were deposited by ion beam sputtering on top of preformed tungsten tips.

Clinical mass spectrometry proteomic diagnosis by conformal predictors.

The paper describes an application of conformal predictors to diagnose breast cancer using proteomic mass spectrometry data provided by Leiden University Medical Center. Unlike many conventional classification systems, this approach allows us not just to classify samples, but add valid measures of confidence in our predictions for individual patients.

[Rational application of the pneumatic tourniquet in the knee joint endoprosthesis].

Two different methods of the pneumatic tourniquet application were used while the knee joint prosthesis in two groups of patients: during the all operation and during the stage of implantation of artificial joint components. It is preferent to reduce the pneumatic tourniquet application duration to realize its positive qualities, to secure optimal adhesion of cement and costal bed and also to make less severe the consequences of intra- and postoperative period. The data obtained trust that the procedure, used by us, constitutes an optimal method of the pneumatic tourniquet application while the knee joint cement prosthesis.

RET/papillary thyroid cancer rearrangement in nonneoplastic thyrocytes: follicular cells of Hashimoto's thyroiditis share low-level recombination events with a subset of papillary carcinoma.

Context: RET/papillary thyroid cancer (PTC) is a marker for papillary thyroid carcinoma, but its specificity has been questioned because of the disputed identification of RET/PTC in Hashimoto's thyroiditis (HT), oncocytic tumors, and other thyroid lesions.

Objective: The objective of this study was to determine 1) whether RET/PTC occurs in nonneoplastic follicular cells of HT, and 2) its recombination rate in thyroid tumors.

Design/patients: Forty-three samples from 31 cases of HT were examined using interphase fluorescence in situ hybridization (FISH) with RET probes spanning the breakpoint region; real-time RT-PCR to quantify RET/PTC1, RET/PTC3, and c-RET transcripts; and RT-PCR after laser capture microdissection to enrich samples for follicular cells.