Confirmation and further regional assignment of aminoacylase 1 (acy-1) on human chromosome 3 using a simplified detection method.

An improve method for the detection of aminoacylase-1 (ACY-1) is described. Data from human-rodent interspecific hybrids confirm the assignment of ACY-1 to human chromosome 3. The most likely site for ACY-1 appears to be 3p21 to 3pter.

Establishment in continuous culture of a T-lymphoid cell line (HD-Mar) from a patient with Hodgkin's lymphoma.

A new cell line, HD-Mar, was established from a pleural effusion of a patient with Hodgkin's lymphoma. Formation of E rosettes, sensitivity to anti-T serum, elevated terminal deoxynucleotidyl transferase activity, presence of T-cell and the common ALL membrane antigens, morphology, and cytochemical staining indicate that the HD-Mar line is of thymic derivation. Absence of any immunoglobulin determinants, the lack of EBNA or any other EBV-associated antigen or function are also characteristics associated with established T-cell-derived lymphoma cell lines.

Carbohydrate of the factor VIII/von Willebrand factor in von Willebrand's disease.

We have examined the plasma Factor VIII/von Willebrand factor (FVIII/vWF) molecule from 16 patients with von Willebrand's disease, and have found no evidence of a significant decrease of carbohydrate content in 15 of these patients. FVIII/vWF was isolated by preparative counter immunoelectrophoresis directly from plasma using antibody to Factor VIII-related antigen, reduced in sodium dodecyl sulfate in the presence of urea, and electrophoresed in 5% polyacrylamide gels to separate the FVIII/vWF subunit from other proteins. Duplicate gels were stained by either the periodic acid-Schiff (PAS) reaction or by Coomassie Brilliant Blue G250.

Ataxia telangiectasia: chromosomal stability in continuous lymphoblastoid cell lines.

Chromosomal breakage in peripheral lymphocytes, cultured fibroblasts and long-term lymphoblastoid cell lines was investigated in five hitherto undescribed patients with ataxia telangiectasia (AT). Increased chromosomal instability was observed in lymphocytes and fibroblasts, and clones possessing a Dq+ marker were observed. Breakage rates were significantly higher in the fibroblasts than in the lymphocytes of AT patients or in similar tissues from patients with Bloom syndrome or Fanconi anemia.

Instrumentation for placement of vitreous carbon endosseous implants.

The instruments required for placement, stabilization, and restoration of vitreous carbon endosseous implants are conventional in character and readily available. With the exception of extra-long burs for socket preparation, all instruments are normally available in dental offices. The surgery instruments should be rigorously sterilized prior to use, including the dental handpiece, burs, suction tip, etc.

[Orientation studies on the stability of metal-ceramic crowns made from new materials].

Materials for metal-ceramic crowns have been developed further in the last few years and the use of this type of crown increases steadily. Numerous investigations concern themselves with binding and other properties of this material as well as with its favourable moulding capacity. The present investigation is oriented towards establishing the order of magnitude and limits of confidence of fracture resistance which has to be reckoned with when using a metal-ceramic crown in the mouth.

[Statistical considerations on the evaluation of the Krogh-Poulsen clinical functional analysis].

361 patients, who five years previously were supplied with removable partial protheses, were submitted to Krogh-Poulson clinical function analyses. The test showed no suggestion of functional disturbances in only 12% of patients. Investigations showed that the test should be improved as to reliability, objectivity and validity before being used as a screening procedure in practice.

[Comparison of cast and metal-ceramic veneer crowns; results of a follow-up study in wearers of partial prostheses].

The results of follow-up examinations of 635 gold cast and 359 metal ceramic veneer crowns inserted primarily for caries prophylaxis and static reasons in patients with partial dentures on the average 4 1/2 years ago are reported. All crowns fulfilled their caries-preventing functions well. More than 93% were free of secondary caries, this was especially so when the crown margin was still below the gingiva.

Association of a chromosomal abnormality with lymphocytes having both T and B markers in a patient with lymphoproliferative disease.

The lymphocytes of a patient with leukemic lymphosarcoma were found to have an unusual surface phenotype in that they bound both sheep erythrocytes (a T cell marker) and complement-coated erythrocytes (a B cell marker) but lacked other B cell surface characteristics. Marker chromosomes were present in these cells, but not in other, phenotypically normal cells from the same patient. This case may provide a clue to the chromosomal origin of some lymphocyte surface markers in man.