[Chemico-toxicological study of fluoxetine].

Selected aspects of chemico-toxicological analysis of the tranquillizer drug fluoxetine are described. Optimal conditions for fluoxetin extraction from internal organs and biological fluids (blood, urine) are specified and methods proposed for its detection and quantitation including TLC, UV SPM, and HPLC. The proposed methods were verified using laboratory animals and materials for expert examination.

[The growth dynamics of nucleoli and their silver stainability in the cell cycle of ESK cells in a monolayer culture].

The cell cycle progression of individual pig embryo kidney (PK) cells was followed by time-lapse microphotography. Evidence has been presented that every detachment of cells from the substrate for subculturing leads to nearly a twofold decrease in the average number of nucleoli per nucleus. However, this number is progressively increased with every generation of flattened cells on the substrate.

[The kinetics of the transition to DNA synthesis in the cell cycle of sister cells of the ESK line in culture].

The proliferation kinetics of a cultured pig embryo kidney cell line, PK, was studied by time lapse cinemicrography and 3H-TdR autoradiography. The duration and variability of all phases of the cell cycle was estimated. Evidence is presented that the variation in the cell cycle transit time of both unrelated and sibling cells results mainly from the variation in transit of G1-phase.

[The effect of picolinic acid and iron ions on the proliferation of SPEV cells].

The effect of picolinic acid (PA) on SPEV cell proliferation is found to be different from that on normal and virus transformed NRC cells, and on spontaneously transformed CHO cells. It is shown that SPEV cells are arrested by PA at the end of G1-phase and at the beginning of S-phase and probably in G2-phase of the cell cycle. Ferrous ions remove the G1/S block induced by PA to permit the cell transfer through S-phase.

Ultrastructural changes in nucleoli and fibrillar centers under the effect of local ultraviolet microbeam irradiation of interphase culture cells.

As shown previously, ultraviolet (uv) microbeam irradiation of one of the two mature nucleoli within an interphase cell nucleus causes significant diminution and inactivation of the irradiated nucleolus and compensatory growth and activation of the nonirradiated one. In the present work we describe the results of an ultrastructural study of this phenomenon. The changes in the nucleoli were examined by means of complete series of ultrathin sections obtained from seven irradiated pig kidney cells.

[Gas-chromatographic detection of acetic acid in cadaveric specimens].

Data on Liquid Gas Chromatographic method for detection of the acetic acid after etherification in the cadaveric organs are presented. The results of quantitative acetic acid determination in the cadaveric organs by LGC method after etherification are influenced upon by its "background" values. Acetic acid concentration in the cadaveric organs of subjects who died from the cause other than acetic acid poisoning is within the limits 0.

[Silver staining of the nucleoli of pig embryonic kidney cells during the hyperactivation and inhibition of the synthesis of nucleolar RNA by UV microirradiation].

Silver staining of the nucleoli in pig embryo kidney cells (PK) was studied during the cell cycle and also upon mature nucleoli modifications induced by UV microirradiation. During anaphase only four silver-stained granules were revealed in each daughter set of chromosomes in the four nucleolus-organizing regions (NORs). In the following 1-2 hours, the number of granules in the NORs rapidly increased up to 25-30 per nucleus.

[Ultrastructure of the interphase nucleoli of pig embryonic kidney cells during their compensatory hypertrophy and degradation due to local UV microirradiation].

As shown elsewhere, the ultraviolet (UV) irradiation of one of the two nucleoli of the interphase cell nucleus results in inactivation and degradation of the irradiated nucleus and in the compensatory growth and activation of the nonirradiated one. In the present work we studied the ultrastructure of degraded and hypertrophied nucleoli in PK-cells with the aid of serial ultrathin sections. The compensatory hypertrophy of the nucleoli was shown to be accompanied by a significant increase in the number of fibrillar centers (FC) and a decrease in their linear size compared with the control ones; in the degraded nucleoli, the FCs number went down, while the size of FCs increased.

[The effect of the intracellular level of free iron on cell proliferation in culture].

Dependence of changes in the intracellular free iron content upon cellular proliferation has been studied on mammalian cells in vitro. It has been established that picolic acid (PA)--a natural metal chelating agent of variable valency--inhibits the proliferation of cultivated pig embryo kidney cells (SPEV). Simultaneously the free iron quantity was decreased 2-fold as compared with the norm.

[Immunologic diagnosis of intravascular blood clotting and intravascular thrombus formation among patients with ischemic heart disease].

Rabbit plasmas immune to fibrinogen/fibrin and thrombin, with antibody titres of 1:1024, were obtained by rabbit immunization with fibrinogen and thrombin. Immunologic methods were shown to be capable of detecting thrombinemia and increased fibrinogen/fibrin levels, as well as reduced titres of anti-thrombin and anti-fibrinogen/fibrin antibodies, i. e.

[Effect of isoptin and euphylline on calcium- and carbacholine-induced contractile activity of isolated intestinal strips of rats of various ages].

It has been shown on isolated strips of the large intestine of adult and 2-week-old rats that the potassium depolarized smooth muscle of the adult rat intestine reacts by contractions to less concentrations of Ca2+ as compared to 2-week-old rats. Euphylline blocked the reaction of the adult rat large intestine to Ca2+ more powerfully than isoptin. The effects of the spasmolytics on 2-week-old rats were less demonstrable in view of the unremarkable reaction to Ca2+ itself.

[Variability of the duration of the cell cycle in pig embryo kidney cells in monolayer culture and correlation of the cycle duration in sister cells].

The distribution of generation time of sister cells for the exponentially proliferating monolayer SPEV culture was obtained with time lapse cinemicrographic technique. The distribution is characterized by the average generation time equal to 24.3 hour, with the variation coefficient, asymmetry coefficient and correlation coefficient for sister pair cell being, respectively, 17%, 0.

[Thickness of mammalian cells in culture, the absorption of UV radiation in the cells and the related shift in action spectra].

The thickness of SPEV cells in monolayer with different cell densities was measured by means of 3-dimensional reconstruction from serial vertical sections. No significant changes in the mean cell thickness were detected despite the wide range of volume and cell density variations. UV absorption at different wave-lengths was measured in various sites of single cells.

[Study of the acute and chronic toxicity of a new preparation of the 3,5-dioxopyrazolidine series, butaglionamide, in comparison with amidopyrine and butadione].

An acute and chronic toxicity of butaglionamide, a new drug in the series of 3,5-dioxopyrazolidines, was contrasted against that of amidopyrine and butadion. The data obtained testify to a much lower toxicity of butaglionamide for rats of different age, both with a single and chronic administration.