[Electric activity of the brain at early and late clinical stages of experimental modeling of Parkinson's disease, an impact of hemantane].

Abstract: S.

Objective: To study an influence of the adamantane derivative hemantane on the electrical activity of the brain structures of mice at the early and late (severe) stages of experimental modeling of Parkinson's disease (PD).

Material And Methods: For experimental modeling of PD in C57BL/6J mice, 30 male C57BL/6J mice weighing 25-32 g were systemically (intraperitoneally) administered proneurotoxin MPTP in two modes corresponding to different clinical stages of the disease: 1-methyl-4-phenyl-1.

Impact of Transition Metal Layer Vacancy on the Structure and Performance of P2 Type Layered Sodium Cathode Material.

This study explores the impact of introducing vacancy in the transition metal layer of rationally designed Na[NiRuMn]O (NRM) cathode material. The incorporation of Ru, Ni, and vacancy enhances the structural stability during extensive cycling, increases the operation voltage, and induces a capacity increase while also activating oxygen redox, respectively, in Na[NiVRuMn]O (V-NRM) compound. Various analytical techniques including transmission electron microscopy, X-ray absorption near edge spectroscopy, operando X-ray diffraction, and operando differential electrochemical mass spectrometry are employed to assess changes in the average oxidation states and structural distortions.

Synuclein Proteins in MPTP-Induced Death of Substantia Nigra Pars Compacta Dopaminergic Neurons.

Parkinson's disease (PD) is one of the key neurodegenerative disorders caused by a dopamine deficiency in the striatum due to the death of dopaminergic (DA) neurons of the substantia nigra pars compacta. The initially discovered A53T mutation in the alpha-synuclein gene was linked to the formation of cytotoxic aggregates: Lewy bodies in the DA neurons of PD patients. Further research has contributed to the discovery of beta- and gamma-synucleins, which presumably compensate for the functional loss of either member of the synuclein family.

Corynebacterium spp.: relationship of pathogenic properties and antimicrobial resistance.

Corynebacterium spp. are part of the human microbiome, but can cause the development of inflammatory diseases of various localization. Purpose - to evaluate the relationship between pathogenic properties and resistance to antimicrobial drugs (AMD) of Corynebacterium spp.

Cystatin C and cystatin SN as possible soluble tumor markers in malignant uveal melanoma.

Background: The aim of the study was to determine the concentration of endogenous cystatin C and cystatin SN, as potential tumor biomarkers, in the serum and biological fluids of the eye in both healthy controls and patients with uveal melanoma.

Patients And Methods: The concentration of both cystatins was determined in the intraocular fluid (IOF), tear fluid, and serum of patients with uveal melanoma and compared to baseline measurements in IOF, tears, serum, cerebral spinal fluid, saliva and urine of healthy controls.

Results: The concentration of cystatin C in all the biological matrices obtained from healthy controls significantly exceeded the concentration of cystatin SN and was independent of gender.

Bio-Derived Surface Layer Suitable for Long Term Cycling Ni-Rich Cathode for Lithium-Ion Batteries.

Since Ni-rich cathode material is very sensitive to moisture and easily forms residual lithium compounds that degrade cell performance, it is very important to pay attention to the selection of the surface modifying media. Accordingly, hydroxyapatite (Ca (PO ) (OH)), a tooth-derived material showing excellent mechanical and thermodynamic stabilities, is selected. To verify the availability of hydroxyapatite as a surface protection material, lithium-doped hydroxyapatite, Ca Li (PO ) (OH), is formed with ≈10-nm layer after reacting with residual lithium compounds on Li[Ni Co Al ]O , which spontaneously results in dramatic reduction of surface lithium residues to 2879 ppm from 22364 ppm.

Gifts from Nature: Bio-Inspired Materials for Rechargeable Secondary Batteries.

Materials in nature have evolved to the most efficient forms and have adapted to various environmental conditions over tens of thousands of years. Because of their versatile functionalities and environmental friendliness, numerous attempts have been made to use bio-inspired materials for industrial applications, establishing the importance of biomimetics. Biomimetics have become pivotal to the search for technological breakthroughs in the area of rechargeable secondary batteries.

The age of adult pilocytic astrocytoma cells.

Adult pilocytic astrocytomas (PAs) have been regarded as indistinguishable from pediatric PAs in terms of genome-wide expression and methylation patterns. It has been unclear whether adult PAs arise early in life and remain asymptomatic until adulthood, or whether they develop during adulthood. We sought to determine the age and origin of adult human PAs using two types of "marks" in the genomic DNA.

Effect of Anti-Glutamate Antibodies in Modeled Parkinsonian Syndrome.

We studied the effect of single and repeated intranasal administration of antibodies to glutamate in experimental parkinsonian syndrome induced by injections of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to C57BL/6J mice. Intranasal administration of anti-glutamate antibodies to mice in parallel with administration of MPTP over 10 days alleviated parkinsonian symptoms (oligokinesia and rigidity). In the serum of mice injected with antibodies to glutamate and/or MPTP, the titers of autoantibodies to glutamate and dopamine were higher than in control animals receiving saline.

High-Voltage Stability in KFSI Nonaqueous Carbonate Solutions for Potassium-Ion Batteries: Current Collectors and Coin-Cell Components.

Cu, Al, and 316L stainless steel are the main components of the current collectors and coin-type cells used in the characterization of potassium-ion battery (KIB) materials and are expected to be electrochemically inactive. Herein, their electrochemical stabilities in a nonaqueous potassium-bis(fluorosulfonyl)imide (KFSI)-based electrolyte are investigated. In dynamic- and transient-mode polarization, passivation of each metal is observed to occur below 3.

The landscape of chromothripsis across adult cancer types.

Chromothripsis is a recently identified mutational phenomenon, by which a presumably single catastrophic event generates extensive genomic rearrangements of one or a few chromosome(s). Considered as an early event in tumour development, this form of genome instability plays a prominent role in tumour onset. Chromothripsis prevalence might have been underestimated when using low-resolution methods, and pan-cancer studies based on sequencing are rare.

KTi (PO ) Electrode with a Long Cycling Stability for Potassium-Ion Batteries.

In this work, rhombohedral KTi (PO ) is introduced to investigate the related theoretical, structural, and electrochemical properties in K cells. The suggested KTi (PO ) modified by electro-conducting carbon brings about a flat voltage profile at ≈1.6 V, providing a large capacity of 126 mAh (g-phosphate) , corresponding to 98.

P2-NaMnO by Co Incorporation: As a Cathode Material of High Capacity and Long Cycle Life for Sodium-Ion Batteries.

The P2-NaMnO compound is one of the attractive cathodes for sodium-ion batteries due to its high initial capacity and abundance of Na and Mn elements in nature. The existence of Mn Jahn-Teller ion, however, impedes electrode performance for long term. Here, we challenge to minimize the effect of the Jahn-Teller distortion caused by Mn in the structure, via substitution of Mn by Co in P2-Na[MnCo]O ( = 0-0.

[The sensitivity to antibiotics of Corynebacterium non diphtheriae isolated in hospitals of Rostov-on-Don and the Rostovskaia oblast.].

The purpose of study is to analyze sensitivity of various species Corynebacterium non diphtheriaе to antibacterial pharmaceuticals. The strains of C. non diphtheriae are separated from patients with pathology of respiratory and urogenital tract and also from individuals subjected to preventive examination.

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting.

While CD133 hematopoietic stem cells (SCs) have been proven to provide high potential in the field of regenerative medicine, their low retention rates after injection into injured tissues as well as the observed massive cell death rates lead to very restricted therapeutic effects. To overcome these limitations, we sought to establish a non-viral based protocol for suitable cell engineering prior to their administration. The modification of human CD133 expressing SCs using microRNA (miR) loaded magnetic polyplexes was addressed with respect to uptake efficiency and safety as well as the targeting potential of the cells.

Recent Progress in Stem Cell Modification for Cardiac Regeneration.

During the past decades, stem cell-based therapy has acquired a promising role in regenerative medicine. The application of novel cell therapeutics for the treatment of cardiovascular diseases could potentially achieve the ambitious aim of effective cardiac regeneration. Despite the highly positive results from preclinical studies, data from phase I/II clinical trials are inconsistent and the improvement of cardiac remodeling and heart performance was found to be quite limited.

Mechanisms of stem cell based cardiac repair-gap junctional signaling promotes the cardiac lineage specification of mesenchymal stem cells.

Different subtypes of bone marrow-derived stem cells are characterized by varying functionality and activity after transplantation into the infarcted heart. Improvement of stem cell therapeutics requires deep knowledge about the mechanisms that mediate the benefits of stem cell treatment. Here, we demonstrated that co-transplantation of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) led to enhanced synergistic effects on cardiac remodeling.

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy.

Small antisense RNAs, like miRNA and siRNA, play an important role in cellular physiology and pathology and, moreover, can be used as therapeutic agents in the treatment of several diseases. The development of new, innovative strategies for miRNA/siRNA therapy is based on an extensive knowledge of the underlying mechanisms. Recent data suggest that small RNAs are exchanged between cells in a gap junction-dependent manner, thereby inducing gene regulatory effects in the recipient cell.

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells.

To date, the available surgical and pharmacological treatments for cardiovascular diseases (CVD) are limited and often palliative. At the same time, gene and cell therapies are highly promising alternative approaches for CVD treatment. However, the broad clinical application of gene therapy is greatly limited by the lack of suitable gene delivery systems.

Stem cell registry programme for patients with ischemic cardiomyopathy undergoing coronary artery bypass grafting: what benefits does it derive?

Aims: Standardization of stem cell therapy requires application of appropriate methods to evaluate safety and efficacy, including long-term pharmacovigilance. To accomplish this objective, a long-term registry programme was installed.

Methods And Results: We analysed 150 patients with ischemic cardiomyopathy, who received intramyocardial CD133+ bone marrow mononuclear stem cell treatment combined with coronary artery bypass grafting (CABG) or CABG alone.

ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS.

Aim: Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae.

Materials And Methods: C.pseudodiphtheriticum, C.

Cystatin C as a Marker of Progressing Cardiovascular Events during Coronary Heart Disease.

The role of cystatin C, an inhibitor of cysteine proteases, as an alternative and potent predictor of acute cardiovascular events in coronary heart disease (CHD) patients was examined and compared to that of other markers of cardiorenal abnormalities. The patients with CHD demonstrated elevated serum cystatin C, especially in cases with serious risk of cardiovascular complications. In comparison with other indicators of cardiorenal dysfunction, cystatin C can be viewed as an alternative predictor of cardiovascular complications, although its sensitivity is inferior to that of high-sensitivity C-reactive protein and natriuretic peptide.

[Corynebacterium riegelii as an unusual species separated from female patient with tuberculosis.].

The article describes a clinical case of tuberculosis of of urinary tracts of a female patient with massive discharge of strain Corynebacterium riegelii from urogenital tract. The data of clinical laboratory examination of female patient are analyzed. The characteristics of isolated strain C.

[CORYNEBACTERIUM: FEATURES OF THE STRUCTURE OF THE BACTERIAL CELL].

In a review of the features of the bacterial cells are Corynebacterium structure: characterized by an upper layer, highly organized cell wall, cytoplasmic membrane, cytoplasm, nucleoid. Described in detail the structure of the upper layer containing pili (fimbriae), microcapsule surface proteins - PS-2, DIP1281, 67-72r protein (hemagglutinin), porins, sialidase (neuraminidase). These components are the ability to initiate a serial of Corynebacterium work with the host cell, followed by colonization.