Bioinformatic identification of proteins with altered PTM levels in a mouse line established to study the mechanisms of the development of fibromuscular dysplasia.

Data from a mass spectrometry experiment of a mouse line developed to study the mechanisms of fibromuscular dysplasia and deposited by d'Escamard et al. in ProteomeXchange (PXD051750) have been analyzed. Identification of peptides with post-translational modifications (PTMs) was repeated using more stringent conditions than in the original work.

bacterium association with atherosclerosis and other diseases.

is a genus of spiral-shaped Gram-negative enterohepatic bacteria whose members are capable of causing bacteremia in humans. One of the poorly studied members of this genus is the bacterium . This microorganism was first isolated from human fecal samples in 1984.

Troubleshooting Paracentesis Using POCUS.

Paracentesis is a procedure routinely performed at the bedside in the evaluation and management of ascites. While point of care ultrasound (POCUS) assistance during paracentesis is known to reduce the risk of procedure-related complications, intraprocedural POCUS to overcome commonly occurring issues, such as obstructed flow through the centesis catheter, remain poorly described. In this report, we present two cases in which bowel adhered to the catheter during paracentesis.

[The prediction of SARS-CoV-2 main protease inhibition with filtering by position of ligand].

The paper analyzes a set of equations that adequately predict the IC50 value for SARS-CoV-2 main protease inhibitors. The training set was obtained using filtering by criteria independent of prediction of target value. It included 76 compounds, and the test set included nine compounds.

[Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis].

The experimental results available in the ProteomeXchange database (accession code PXD016538) (Simats et al. (2020) Molecular and Cellular Proteomics, 19(12), 1921-1936) obtained using a comprehensive multi-omics approach were analyzed in mouse blood to identify potential biomarkers of ischemic stroke. Acetylation, methylation, and ubiquitination were considered as post-translational modifications.

Dormancy: There and Back Again.

Many cells are capable of maintaining viability in a non-dividing state with minimal metabolism under unfavorable conditions. These are germ cells, adult stem cells, and microorganisms. Unfortunately, a resting state, or dormancy, is possible for tuberculosis bacilli in a latent form of the disease and cancer cells, which may later form secondary tumors (metastases) in different parts of the body.

[Dormancy: There and Back Again].

Many cells are capable of maintaining viability in a non-dividing state with minimal metabolism under unfavorable conditions. These are germ cells, adult stem cells, and microorganisms. Unfortunately, a resting state, or dormancy, is possible for tuberculosis bacilli in a latent form of the disease and cancer cells, which may later form secondary tumors (metastases) in different parts of the body.

Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.).

Correlations between the morphological features of f lower buds and the developmental stages of the male gametophyte are of great practical interest as a reliable marker that accelerates and simplif ies the selection of appropriate plant material for isolated microspore culture. Microspore culture enables one to quickly obtain many pure lines of different vegetable crops, but it has not yet been widely applied in the melon (Cucumis melo L.).

[The bioinformatic identification of proteins with varying levels of post-translational modifications in experimental ischemic stroke in mice].

The experimental data obtained by Simats A. et al. (Molecular and Cellular Proteomics, 2020, 19(12), 1921-1936) was analysed using a bioinformatic approach.

Methodological approaches for producing doubled haploids in sugar beet and red beet (Beta vulgaris L.).

The in vitro production of doubled haploids is a biotechnological path of an accelerated development of parental lines in F1-hybrid breeding programs. Unlike the traditional inbreeding method requiring 5 to 6 generations to reach a suf-f icient homozygosity of lines, the number of generations to produce pure lines of beet by haploid technologies is reduced to 2. The production of doubled haploids by gynogenesis is the most common biotechnological approach in sugar and red beets.

To the Academician Alexander Spirin 90th Anniversary from Informosomes to the World of mRNP.

The main stages and achievements in the investigation of the fate of mRNA in eukaryotic cells, which began half a century ago with the discovery of informosomes by A.S. Spirin, there are discussed.

Association between occupational exposures and sarcoma incidence and mortality: systematic review and meta-analysis.

Background: Sarcomas are a rare and heterogeneous group of tumors originating from mesenchymal or connective tissue. They represent less than 1% of all adult cancers. The etiology and epidemiology of sarcomas remain understudied and poorly understood.

Aspects of "antigen-antibody" interaction of chicken infectious bronchitis virus determined by surface plasmon resonance.

Authors performed investigation on "antigen-antibody" interaction of chicken infectious bronchitis coronavirus (IBV) by a method based on the surface plasmon resonance (SPR). Presence of space-size effect related to a difference between antigen and antibody particle sizes has been theoretically grounded and experimentally proven. Herewith, the difference between responses of the SPR-sensor to specific and non-specific interactions is considerably less (up to 6.

mRNPs: Structure and role in development.

In eukaryotic cells, mRNA molecules are coated with numerous RNA-binding proteins and so exist in ribonucleoproteins (mRNPs). The proteins associated with the mRNA regulate the fate of mRNA, including its localization, translation and decay. Before activation of translation, the mRNA does not display any template functions-it is masked.

Label-Free Single Cell Viability Assay Using Laser Interference Microscopy.

Laser interference microscopy (LIM) is a promising label-free method for single-cell research applicable to cell viability assessment in the studies of mammalian cells. This paper describes the development of a sensitive and reproducible method for assessing cell viability using LIM. The method, based on associated signal processing techniques, has been developed as a result of real-time investigation in phase thickness fluctuations of viable and non-viable MCF-7 cells, reflecting the presence and absence of their metabolic activity.

Assessment of thirty-day readmission rate, timing, causes and predictors after hospitalization with COVID-19.

Background: There are limited data on the characteristics of 30-day readmission after hospitalization with coronavirus disease 2019 (COVID-19).

Objectives: To examine the rate, timing, causes, predictors and outcomes of 30-day readmission after COVID-19 hospitalization.

Methods: From 13 March to 9 April 2020, all patients hospitalized with COVID-19 and discharged alive were included in this retrospective observational study.

Critical Review of Existing MHC I Immunopeptidome Isolation Methods.

Major histocompatibility complex class I (MHC I) plays a crucial role in the development of adaptive immune response in vertebrates. MHC molecules are cell surface protein complexes loaded with short peptides and recognized by the T-cell receptors (TCR). Peptides associated with MHC are named immunopeptidome.

The development of Ac isotopic generator.

The paper describes a method of recovery of Ac tracer from an old thorium salt through sequential separation of Ra by sorption onto the MnO-CTA disk from thorium solution followed by Ac/Ra separation using the Axionit 507 extraction chromatographic resin. It was shown that the optimal conditions of radium separation from thorium nitrate solution are pH 4.7-6.

Xvent-2 expression in regenerating tails.

The tail of tadpole is an excellent model for appendage regeneration studies. We analyzed the distribution pattern of the transcription factor Xvent-2 mRNA and protein in the beginning of the regeneration of tadpole tail stumps after amputation. We revealed the emergence of Xvent-2 mRNA and protein in regeneration bud during the first day after amputation.

[Plant Stem Cells].

A brief review of current data on the molecular biology of stem cells forming meristems and differentiating into various organs of angiosperms is presented. Different primary and secondary meristems are compared. The interactions of some hormones, regulatory gene networks, and signaling pathways in different types of meristems are described.

Decontamination of seawater from Cs and Sr radionuclides using inorganic sorbents.

In this work, we have studied sorption of Сs and Sr radionuclides from seawater under batch conditions by ferrocyanide sorbents based on hydrated titanium and zirconium dioxides (Т-35, NPF-HTD), clinoptilolite and glauconite (NPF-GL, NPF-CL) natural aluminosilicates, zirconium phosphate (T-3A), modified hydrated titanium dioxide (T-3K) as well as by manganese dioxide based on hydrated titanium dioxide (MD-HTD). Isotherms of sorption and dependences of cesium distribution coefficients on salt content and calcium concentration were obtained. Distribution coefficients of cesium and strontium were calculated.

[Neural Crest-An Unusual Population of Embryonic Cells].

The neural crest (NC) in embryos of vertebrates represents a cell population formed at the border of the neural plate. These cells retain pluripotency, express a set of specific markers, and become multipotent upon their migration away from the neural tube to give rise to numerous derivatives. The genes specific for vertebrate NC appeared in evolution long before vertebrates.

Incidence of Ventral Hernia Repair after Open Abdominal Aortic Aneurysm and Open Aortofemoral or Aortoiliac Bypass Surgery: An Analysis of 17,594 Patients in the State of New York.

The purpose of our study was to evaluate the rate of ventral hernia repair (VHR) after open abdominal aortic anneurysm in New York State compared with the rate of VHR after open abdominal aortic bypass procedures. The Statewide Planning and Research Cooperative System database was queried for all abdominal aortic aneurysm (AAA) and bypass procedures performed between 2000 and 2010. Social security death index was used to identify patients who died.

The ved protein patterning in zebrafish embryos.

Homeobox transcription factors play an essential role in cells differentiation. The function is realized by the proteins (not by the mRNA) and it is necessary to pay more attention to the protein patterns. In this study we were the first to obtain antibodies against the ved protein, tested their specificity by Western-blot analysis and performed a whole mount immunostaining of zebrafish embryos.

ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner.

Hydrogen peroxide (HO) increases paracellular permeability of Madin-Darby canine kidney (MDCK) cells, but the mechanism mediating this effect remains unclear. Treatment of MDCK cells with HO activated ERK 1/2. Inhibition of ERK 1/2 activation blocked the ability of HO to increase paracellular permeability.