[Regional differentiation of demographic dynamics in Russia and the role of healthcare].

The article examines the role of the processes of natural and migration movement of the population in changing the total population of the regions of Russia for more than two decades of the XXI century. Six possible combinations of these processes are proposed, which result in either an overall increase in the population or a reduction. During the period under review, various scenarios in a number of regions had fairly stable trends - a constant decline in the population or a stable increase in it.

Use of Virtual Reality Techniques to Rehabilitate Military Veterans with Post-Traumatic Stress Disorder (Review).

Post-traumatic stress disorder (PTSD) is a mental and behavioral disorder developing after a traumatic event like participation in combat activities. Objective diagnosis of combat PTSD and effective rehabilitation of war veterans is a current multifaceted problem with particularly high social costs. This review aims to evaluate the potential of virtual reality technique used as exposure therapy tool (VRET) to rehabilitate combat veterans and service members with PTSD.

Infrared Analysis and Effect of Nitrogen and Nitrous Oxide on the Glass Transition of Methanol Cryofilms.

Methanol plays an important role in studying the structure and dynamics of hydrogen bonds in alcohols and other physiologically important compounds in the condensed state. The physical vapor deposition method and two-beam interferometry make it possible to study the structure of polyatomic molecules, the nature and character of intermolecular interactions, and the internal structure of various compounds. Thus, it becomes possible to analyze changes in the internal structure of substances near the points of their phase transitions and glass transitions at ultralow temperatures.

[The demographic aging of population: regional trends in Russia].

The article considers evolution of research and views on challenges and menaces of demographic aging process in the works of national and foreign scientists. The characteristics of this process in regions of the Russian Federation consist in special diversity of social economic, climatic, geographical and mental conditions in which demographic processes proceed. This determined availability of various types and stages of demographic aging in regions of the Russian Federation as well as opportunities to temper negative consequences of this process.

Optical Studies of Thin Films of Cryocondensed Mixtures of Water and Admixture of Nitrogen and Argon.

The interaction of host molecules with water molecules is of primary importance in astrophysical and atmospheric studies. Water-binding interactions continue to attract a broad interest in various fields, especially those related to the formation of assembly structures. Using the physical vapor deposition (PVD) method and a two-beam interferometer with a wavelength of 406 nm, the refractive indices of thin films of a water and nitrogen (argon) mixture were calculated in the range from 15 to 35 K.

Effect of Bone Marrow Allotransplantation on Neuron-Specific (NSE) and Histamine-Containing Cells of Lymph Nodes.

The histamine content in bioamine-containing cells and the content of NSE cells of the lymph nodes were studied in rats in 40 min and in 2 and 4 h after bone marrow allotransplantation by using the cross luminescence-histochemical method and immunohistochemical method, respectively. Within 2 h after allotransplantation of the bone marrow, a significant increase in histamine content in bioamine-containing cells and an increase in the number of NSE cells in the period were observed. Both APUD and NSE+ cells were found to be heterogeneous by staining and luminescence.

Effect of Bone Marrow Autotransplantation on the Numerical Population of Tryptase-Positive Mast Cells, Heparin Sulfation Degree, and Histamine Content.

We studied the effect of bone marrow autotransplantation on the morphofunctional properties and numerical population of mast cells. The experiments were performed on 4-monthold male mice. The animals received an injection of a suspension of bone marrow obtained from the femoral epiphyses of these animals into the caudal vein.

The New Pharmaceutical Compositions of Zinc Oxide Nanoparticles and Triterpenoids for the Burn Treatment.

We studied oleogels containing zinc oxide nanoparticles (ZnO NPs) and lupane triterpenoids in sunflower oil for the treatment of burns. The modification of ZnO was carried out by treatment with alcohol solutions of betulin, betulonic acid, betulin diacetate and betulin diphosphate. The properties of modified ZnO NPs were studied by powder XRD (average sizes of 10-20 nm), FTIR (νZnO 450 cm), UV-vis (345-360 nm), and blue-violet emission (380-420 nm).

Serotonin-Containing Cells in the Primary Organ of Hemopoiesis after Autologous Bone Marrow Transplantation.

Serotonin-containing cytostructures of mouse bone marrow after autotransplantation were studied using Falk-Hillarp luminescent histochemical method, and expression of antiapoptotic marker was determined by the immunohistochemical reaction. Autotransplantation of the bone marrow in mice led to an increase in the number of mast cells; in 40 and 120 min after autotransplantation, serotonin content in mast cells increased by 12 and 23%, respectively, and in hemopoietic cells of the bone marrow - by 24 and 78%, respectively, which affected the early stages of proliferation and differentiation of hemopoietic cells. The mitotic index in bone marrow smears at these terms increased by 3.

Effects of Bone Marrow Autotransplantation on the Bone Marrow Neurotransmitter Structures.

Morphological structures involved in local regulation of tissue processes and the neurotransmitters they produce are assumed to play an important role in the pathogenesis of oncological diseases. We studied the dynamics of neurotransmitter cell counts and distribution of catecholamines and serotonin in them and analyzed proliferative activity of hemopoietic cells in the bone marrow of mice after bone marrow autotransplantation. The bone marrow of male mice was studied by fluorescent and immunohistochemical methods after injection of the bone marrow from the same mouse into the caudal vein.

Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation.

The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation.

Betulin-3,28-diphosphate as a Component of Combination Cytostatic Drugs for the Treatment of Ehrlich Ascites Carcinoma In Vitro and In Vivo Experiments.

The activity of betulin-3,28-diphosphate (BDP) in combination with the cytostatics such as 5-fluorouracil (5-FU) and hydrazine sulfate (HS) was demonstrated by using the transplanted Ehrlich ascites carcinoma (EAC) in mice. The dose-dependent effect of combination drugs BDP + HS and BDP + 5-FU was revealed by in vitro experiments on rats. The synergetic effect of HS and BDP on oxidative stress and energy metabolism was established.

[An influence of interictal anxiety on coping and self-control strategies for epileptic seizures in patients with temporal lobe epilepsy].

Aim: A comparative analysis of coping and self-control strategies for epileptic seizures in temporal lobe epilepsy patients with and without interictal anxiety.

Material And Methods: Authors evaluated interictal anxiety (IA) with HADS, strategies for seizure control with CBES-SC, coping-style with BriefCOPE scale and quality of life with QOLIE-31-P scale in 45 patients with temporal lobe epilepsy (TLE). The control group consisted of 86 healthy individuals.

[Nitric oxide: from the mechanism of action to pharmacological effects in cerebrovascular diseases].

The article presents the data of studies of nitric oxide (NO) in the pathogenesis of cerebrovascular diseases. It is emphasized that endothelial dysfunction contributes to the formation of cerebrovascular diseases. Generalized data on preparations with endothelioprotective effect, as well as own data on the use of the preparation 'Divaza' in patients of middle and advanced age with chronic cerebrovascular disease are given.

Use of Cre/loxP recombination to swap cell binding motifs on the adenoviral capsid protein IX.

We used Cre/loxP recombination to swap targeting ligands present on the adenoviral capsid protein IX (pIX). A loxP-flanked sequence encoding poly-lysine (pK-binds heparan sulfate proteoglycans) was engineered onto the 3'-terminus of pIX, and the resulting fusion protein allowed for routine virus propagation. Growth of this virus on Cre-expressing cells removed the pK coding sequence, generating virus that could only infect through alternative ligands, such as a tyrosine kinase receptor A (TrkA)-binding motif engineered into the capsid fibre protein for enhanced infection of neuronal cells.