[Problem of bioterrorism under modern conditions].

It is practically impossible to discuss the problem of bioterrorism (BT) and to develop effective programs of decreasing the losses and expenses suffered by the society from the BT acts without evaluation of the threat and prognosis of consequences based on research and empiric data. Stained international situation following the act of terrorism (attack on the USA) on September 11, 2001, makes the scenarios of the bacterial weapon use (the causative agents of plague, smallpox, anthrax, etc.) by international terrorists most probable.

[Evaluation of microecology of colonic contents in patients with chronic pancreatitis].

The paper deals with microbiocenosis in the colonic content in 60 patients with chronic pancreatitis during its exacerbation. An analysis has indicated that in the colonic content there are the following microorganisms: Bifidobacterium sp., 10(8)-10(10) CFU/g; Lactobacillus sp.

[Results of basic and applied investigations on oral immunization against smallpox ].

The results of fundamental and applied investigations on the development and trial of the oral administration of smallpox vaccine and live recombinant smallpox-hepatitis vaccine (Revax VT) in tablets are summarized. In comparative experiments on animals (rabbits, monkeys and guinea pigs) and human immunization the oral smallpox vaccine in tablets was shown to ensure equal effectiveness and greater safety in comparison with traditional smallpox vaccine for skin application. The study confirmed the natural and physiological character of oral immunization as a result of direct contact of immunogen with the mucous membrane of the digestive tract--an essential immunocompetent organ of the lymphoid system.

[State of normal intestinal microflora in preschool children living in the ecologically adverse region].

In children living in an ecologically unfavorable area the quantitative content of bifidobacteria and enterococci appeared to be considerably decreased while the level of sulfate-reducing clostridia, on the contrary, elevated. The suppression of bifidoflora leads to decreased immune responsiveness that promotes different somatic diseases. Bronchitis, pneumonia, tonsillitis, pharyngitis, otitis, allergic diseases, diseases of digestive organs, acute respiratory virus infections are more often registered in children living in ecologically unfavorable Central district than in children living in the ecologically favorable Lenin district.

[Intestinal microbiocenosis at the critical periods of child development].

The bacteriological study of fecal specimens obtained from 595 children at different critical periods of the development of their immune system was carried out. The comparison of intestinal microflora in children at the critical periods of the development of their immune system revealed that in children under 1 year considerable changes in the microflora composition were observed during the first 6 months of life. These changes, most pronounced in children aged 1 month and 3-6 months, were characterized by decreased level of indigenous microflora and an increase in the qualitative and quantitative content of aerobic microflora with the representatives of the family Enterobacteriaceae playing the dominating role.

[Comparative study of the effectiveness of HBcAg presentation to the immune system using attenuated Salmonella strains, serovars S.enteritidis and S.typhimurium].

In this work the results of obtaining HBcAg-producing attenuated Salmonella strains, serovars S. enteritidis and S. typhimurium, and their comparative study is presented.

[Transcription map of the 13q14 region, frequently deleted in B-cell chronic lymphocytic leukemia patients].

Deletions in the region located between the STS markers D13S1168 and D13S25 on chromosome 13 are the most frequent genomic changes in patients with B-cell chronic lymphocytic leukemia (B-CLL). After sequencing of this region, two novel candidate genes were identified: C13orf1 (chromosome 13 open reading frame 1) and PLCC (putative large CLL candidate). Analysis of the repeat distribution revealed two subregions differing in composition of repetitious DNA and gene organization.

[Production of stable Bacillus thuringiensis suspensions in an electromagnetic apparatus].

The experiment demonstrated the podibility of obtaining stable suspensions of B. subtilis, a microorganism used as an experimental model, in an electromagnetic apparatus with a bilateral inductor. The optimum conditions of obtaining such suspensions were chosen, these conditions excluding the inactivation of the bacteria used as a model in the course of the process.

[Formation of intestinal microflora in children during the first year of their life].

In 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. The study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. During this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance.

[Persistence of Escherichia coli recombinant strains in experimental animals].

Recombinant E. coli strains, obtained by gene engineering techniques and capable of producing human alpha-interferon and HIV proteins, were studied. The cultures under study were completely eliminated from the body of experimental animals (mice) in 48 hours, and generalization of the infectious process took place.

[Age-dependent characteristics of intestinal microbiocenosis in Kemerovo residents].

In 873 residents of Kemerovo aged 1-60 years and older the microflora of the large intestine was studied. The study revealed that in all examined subjects dysbiotic changes in the composition of normal microflora were observed. More profound disturbances of microbiocenosis were found in children aged 1-2 years, in adolescents and in adults over 60 years of age.

[Vaginal microflora in girls of prepuberal age].

The bacteriological study of vaginal microflora in 24 clinically healthy girls aged 7-8 years was carried out. Two criteria of normal vaginal biocenosis in girls of prepuberal age were proposed. The proportion of hemolytic bacteria in the vagina of a girl of prepuberal age could reach 100% of all microflora, provided the total bacterial count (TBC) exceed average values.

[Impaired interferon status in children with acute respiratory infection and its correction with bifidumbacterin-forte].

The state of interferon status was studied in 46 hospitalized children: 33 patients with complicated forms of acute respiratory virus infection (ARVI), such as pneumonia, bronchitis, etc., and 13 patients with vegetovascular dystonia (used as a comparison group). The study revealed that in patients with acute infections of the upper and lower respiratory tract considerable changes in their interferon system were registered.

[Method of production of the dried highly dispersed vaccine of Newcastle disease virus].

Experimental batches of dried dust-like vaccine on the basis of Newcastle disease virus, strain La Sota, have been prepared. The technology of the preparation of the vaccine includes the lyophilization of the technical fluid of the culture under study, milling dried biomass and mixing the pulverized biomass with excipient. As revealed in the process of this work, lyophilization ensures high concentration of the virus in dried biomass and its pulverization, a high content of the target fraction of biomass particles in the vaccine preparation with the moderate inactivation of the bioagent.

[Opportunistic microorganisms in purulent inflammatory otolaryngologic diseases and meningitis].

The contamination of clinical specimens material, obtained from patients with otolaryngology inflammatory processes and purulent meningitides in the Moscow region, has been studied. Etiologically significant causative agents dominating in different purulent inflammatory diseases have been established. As revealed in this study, in the Moscow region the leading causative agents of purulent inflammatory otolaryngology deseases and meningitides are coagulase-negative ataphylococci, Escherichia coli, meningococci, pyogenic streptococci and fungi of the genus Candida.

[Taxonomy and classification of Rickettsiae].

During the recent 20 years the improvement of the methodological research basis including the molecular and genetic analysis of microorganisms parasites of eukaryotic cells, led to considerable changes in the taxonomy and classification of rickettsiae. This problem was not discussed in the Russian literature. At present the term "rickettsiae" conventionally embraces the group of microorganisms of the class Proteobacteria, nominated as species belonging to the genera Rickettsia, Orientia, Ehrlichia, Coxiella and Bartonella.

[Microflora in ENT and CNS diseases in patients living in the Moscow region].

Patients with ENT and CNS inflammation living in the Moscow Region have been examined for pathogenic contamination of relevant clinical samples. The microflora changes were followed up, pathogenic agents of high and moderate priority were identified. It is shown that for Moscow Region, ENT and CNS purulent inflammation is associated primarily with coagulase-negative staphylococci, E.

[Microbiological aspect of balanced nutrition].

Analysis of modern resources of normal human microflora correction has shown an importance of microbiological aspect in principles of a balanced feeding for realization of the optimum mechanism of normal human microflora maintenance.

[Possibility of participation of bacterial factor in the correction of histamine metabolism in the body].

Microbial factor belongs a significant role in mechanisms of regulation of contents free histamine in the organism. Magnified amounts this mediator connected with the condition of microbial ecology, can stimulate a number of pathophysiological effects. This dictates need of further studying dug autoflora master in processes of accumulation free histamine in the organism and their correction.