Active tapered double-clad fiber with low birefringence.

We addressed the problem of a state of polarization (SOP) drift caused by heating under intense clad pumping in different types of active tapered double-clad fibers. We investigated experimentally the variations of the SOP and degree of polarization (DOP) under clad pumping in polarization-maintaining (PANDA type) and regular (non-PM) Yb-doped double-clad large mode area tapered fibers. We discovered that the birefringence of active fibers is highly dependent on the launched pump power.

[Expression of early hematopoietic markers in cord blood and mobilized blood].

G-CSF mobilized peripheral blood and cord blood are major sources of hematopoietic progenitor cells. These cells are characterized by the expression of "early" antigens. We have evaluated the coexpression of hematopoietic cell markers CD34, CD133, CD90, CDCP1, CD117 and activation antigen CD38 using multicolor flow cytometry.

[Microtubules suppress blebbing and stimulate lamellae extension in spreading fibroblasts].

We compared spreading of Vero fibroblasts when microtubules were depolymerized or stabilized. After initial attachment cells start blebbing that continues for different time and abruptly transfers into spreading. After spreading initiation, most cells spread in an anisotropic manner through stochastic formation of lamellipodia.

[Imaging of surface cell antigens on the tumor sections of lymph nodes using fluorescence quantum dots].

The usefulness of quantum dots for the immunofluorescent detection of surface antigens on the lymphoid cells has been studied. To optimize quantum dots detection we have upgraded fluorescent microscope that allows obtaining multiple images from different quantum dots from one section. Specimens stained with quantum dots remained stable over two weeks and practically did not bleach under mercury lamp illumination during tens of minutes.

[Nine-year experience in the treatment of patients with diffuse large B-cell lymphosarcoma].

Aim: To ascertain indications to standard (CHOP-21/R-CHOP-21) and intensive (mNHL-BFM-90) treatment in patients with diffuse large B-cell lymphosarcoma (DLBCL) with involvement of lymphoid organs.

Material And Methods: The trial, performed from January 2002 to December 2010, enrolled 139 DLBCL patients with affected lymph nodes (LN), tonsils, spleen, bone marrow (BM). The diagnosis was made according to WHO criteria.

[Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry].

Quantum dots (QD) nanocrystals consisting of CdSe core with ZnS shell are a novel class of fluorophores with tremendous potential in microscopy and cytometry techniques. The unique optical features of Qdots, namely, high photostability and extinction coefficient, wide absorption and narrow emission spectra, and large Stokes shift make them desirable fluorescent tags for diverse biomedical applications. Applications of this novel technology in microscopy and cytometry produce reliable multicolor specimens due to increased photostability, ability for multiplexing and narrow emission spectra of nanocrystals.

[Molecular dynamics method for proteins with ionization-conformation coupling and equilibrium titration].

A new realization of the constant-pH molecular dynamics simulation method is proposed. Molecular dynamics simulation is performed for a protein in the most probable ionization microstate of the current conformation taking into account the potential of mean force of protein molecule in the water-proton bath in equilibrium titration conditions (MD-pH-ET). It is shown that: 1) the optimal one is the simulation of the protein in the most probable ionization for a given conformation, taking into account the correction ionization potential of mean force, which results in the librium ensemble of ionization states; 2) new method MD-pH-ET allow one to carry out an optimization of protein structure and the total free energy of a protein in the aqueous solution at constant pH, and to calculate the pH-dependent properties.

[Plasma catecholamines in consciousness recovery in patients with severe traumatic brain injury].

Changes in plasma noradrenalin (NA) and dopamine (DA) levels were evaluated in the stages of consciousness recovery in patients with severe traumatic brain injury with and without deep brain structure damage. Forty-eight patients (36 men and 12 women), aged from 12 to 56 (31,9±10,9) yeas, were enrolled in the study. Two variants of catecholamine (CA) changes were found: 1) a CA-dissociation that was oppositely directed to NA and DA changes was observed in unconsciousness; 2) a CA-dissociation (unidirectional NA and DA changes) that was observed after the restoration of consciousness.

[GUI-BioPASED program for molecular dynamics modelling of biopolymers with a graphical user interface].

There are several software packages that allow the user to model main types of biopolymers with molecular dynamics methods. However, the work with most of these packages is quite complicated, especially for non-experts in computational structural biology. We have created GUI-BioPASED, a Web-based GUI for molecular dynamics software BioPASED, to partially automate the tasks usually responsible for the majority of errors.

[Diffuse large B-cell lymphoma with primary involvement of mediastinal lymph nodes: diagnosis and treatment].

Aim: To diagnose diffuse large B-cell lymphosarcoma (DLBCLS) with primary involvement of the mediastinal lymph nodes (LN) and to evaluate the efficiency of aggressive polychemotherapy (PCT).

Subjects And Methods: The study included 15 patients (6 men and 9 women aged 18 to 70 years; median 38 years) followed up at the Hematology Research Center, Russian Academy of Medical Sciences, in 2004 to 2009. Three and 12 patients had Stages II and IE DLBCLS, respectively.

[Radial-organized microtubules provide maintenance of the cell shape and more effective intercellular transport than in the case of free microtubules].

Microtubules take part in very different cell processes including cell polarization and migration, intercellular transport and some others. Therefore the microtubules spatial organization is crucial for normal cell behaviour. Fibroblasts have radial microtubule array consisting of microtubules running from the centrosome.

[A morphometric analysis of the nuclei and nucleoli in tumor cells in lymphogranulomatosis, diffuse large B-cell lymphoma and anaplastic large cell lymphoma].

Aim: To make a comparative morphometric analysis of the nuclei and nucleoli of tumor cells in lymphogranulomatosis (LGM), diffuse large B-cell lymphoma (DLBCL) and anaplastic large cell lymphoma (ALCL) for differential diagnosis of these lymphomas.

Material And Methods: Biopsy material (lymph node biopsies) was frozen in hexane, fixed and stained, then microscopic pictures were made.

Results: Mean area of tumor cell nuclei in LGM was 97.

[The spatial organization of centrosome-attached and free microtubules in 3T3 fibroblasts].

Microtubules spatial organization is essential for different cellular processes to proceed normally. It is supposed traditionally, that the fibroblasts have radial microtubule array consisting of long microtubules running from the centrosome. However, the detailed analysis of the microtubule array in the internal cytoplasm has never been performed.

[Correlation of lipid spectrum with red blood cells izoantigene polymorphism and nutritional status of isetski believers].

In genetically homogeneous groups food habits associate with a number of food substances which act with food stuffs, and render essential influence on a lipid spectrum wheys of blood. Thus can influence character of current not only not infectious, but also on infectious inflammatory diseases which associate with secondary immune.

[Features of immune status among native population of Yamal in the conditions of Europeanization of traditional food].

The destruction of nutrition traditional structure and imbalance of food allowance by the basic kinds of plastic and energy materials impose a negative mark on efficiency of immune protection of north Aboriginals.

[Treatment of life-threatening airway obstruction in patients with malignant lymphomas].

Case histories of patients with hemoblastosis admitted to a hospital for acute respiratory failure due to tumor-induced obstruction were retrospectively analyzed. The causes of obstruction, antitumor therapy, methods for provision of airway patency, and major critical syndromes were analyzed. Ten patients with life-threatening tumor-induced airway obstruction were hospitalized from 1995 to 2007.

[Efficacy of therapy of different variants of anaplastic large T-cell lymphomas].

Aim: To compare efficacy of NHL-BFM-90 and CHOP-like courses in the treatment of anaplastic large cell lymphoma (ALCL).

Material And Methods: Twenty-two patients with ALCL participated in the study. The diagnosis was made basing on the findings of clinical, device, morphological, immunohistochemical and molecular-genetic examinations with application of a panel of monoclonal antibodies to CD30, ALK, CD3, CD4, CDS, CD7, CD34, CD15, CD68, CD20, CD45RO, CD45RA, Ki-67.

[High-dose therapy of Burkitt's lymphoma in patients over 40 years of age].

Aim: To analyse efficacy and tolerance of high-dose polychemotherapy (PCT) of Berkitt's lymphoma (BL) in patients aged over 40 years.

Material And Methods: High-dose PCT was given to 6 BL patients aged 41-56 years (median 48.1 years).

[On the relation of the microtubule length and dynamics: boundary effect and properties of an extended radial array].

In interphase cells, microtubules (MT) are long and form extended radial array. The length of individual MTs in living cells exhibits substantial stochastic fluctuations while the average length distribution in a cell remains nearly constant. We present a quantitative model that describes relation of the MT length and dynamics in the steady state in the cell using the minimal set of parameters (cell radius, tubulin concentration, critical concentration for plus end elongation, and the number of nucleation sites).

[Molecular modeling of positioning of human release factor eRF1 relative to mRNA stop-codon explains a proximity of the eRF1 C-domain to stop-codon in ribosomal complex].

A properties of atomic models of structure of eukaryotic triple complex eRF1 . mRNA . tRNAPhe containing human class-1 polypeptide release factor eRF1 at the A-site of human 80S ribosome, mRNA and P-site tRNAPhe, obtained before, are considered.

[Expression of human serum albumin in metylotrophic yeast Pichia pastoris and its structural and functional analysis].

The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.

[Primary diffuse large B-cell lymphosarcoma of the spleen].

Aim: To investigate characteristics of the course and efficacy of treatment of diffuse large B-cell lymphosarcoma (DLBL) with primary lesion of the spleen.

Material And Methods: From 1998 to 2006, primary splenic lesion was registered in 15 of 120 patients with DLBL and affected lymph nodes (LN), spleen and Waldeyer's ring. The diagnosis was made according to WHO criteria.