Investigation of growth and sporulation during larval rearing.

Insects are increasingly used as an alternative protein source for feed and food production. One of the main biological hazards associated with edible insects is the bio-accumulation of foodborne pathogenic microorganisms. In this study, the interaction of larvae of the black soldier fly (BSFL, (L.

Cross-sectoral genomic surveillance reveals a lack of insight in sources of human infections with Shiga toxin-producing , the Netherlands, 2017 to 2023.

Shiga toxin-producing (STEC) is a zoonotic pathogen associated with illness ranging from mild diarrhoea to haemolytic uremic syndrome (HUS) or even death. Cross-sectoral data sharing provides an opportunity to gain insight in reservoirs and sources of human infections and starting points for pro-active measures. Nevertheless, phylogenetic clustering of STEC strains from animals, food and human cases is low in the Dutch surveillance system.

Bringing the autistic lifeworld to supportive technology design: an enactive approach.

Supportive technologies for autistic individuals are promising in principle, yet their uptake remains limited. Critics argue that in current designs of supportive technologies, autism is mostly framed as a 'disorder' whose limitations can be pragmatically compensated for. To increase uptake, designers should get a better handle on how to incorporate the full richness of the autistic experience into the design process.

A new approach and insights on modelling the impact of production diseases on dairy cow welfare.

Animal welfare is becoming an important consideration in animal health-related decision-making. Integrating considerations of animal welfare into the decision-making process of farmers involves recognising the significance of health disorder impacts in relation to animal welfare. Yet little research quantifies the impact, making it difficult to include animal welfare in the animal health decision-making process.

N-Glycosylation-dependent regulation of immune-specific tetraspanins CD37 and CD53.

Tetraspanin proteins play an important role in many cellular processes as they are key organizers of different receptors on the plasma membrane. Most tetraspanins are highly glycosylated at their large extracellular loop; however, little is known about the function of tetraspanin glycosylation in immune cells. In this study we investigated the effects of glycosylation of CD37 and CD53, two tetraspanins important for cellular and humoral immunity.

Combining Whole Genome Sequencing Data from Human and Non-Human Sources: Tackling Outbreaks.

() is ubiquitous in nature and known for its ability to contaminate foods during production processes. Near real-time monitoring of whole genome sequences from food and human isolates, complemented with epidemiological data, has been used in the Netherlands since 2019 to increase the speed and success rate of source finding in the case of (active) clusters. Nine clusters with 4 to 19 human cases investigated between January 2019 and May 2023 are described.

Virulence and antimicrobial resistance of Shiga toxin-producing Escherichia coli from dairy goat and sheep farms in The Netherlands.

Aims: The aim of our study was to investigate the virulence and resistance of STEC from small ruminants farms in The Netherlands. Moreover, the potential transmission of STEC between animals and humans on farms was evaluated.

Methods And Results: From 182 farms, in total, 287 unique STEC isolates were successfully recovered from animal samples.

New insights into the epidemiology of - A cross-sectoral retrospective genomic analysis in the Netherlands (2010-2020).

Introduction: Listeriosis, caused by infection with (), is a relatively rare but severe disease with one of the highest mortality rates among bacterial foodborne illnesses. A better understanding on the degree of clustering, the temporal distribution of the clusters, and their association with the various food sources is expected to lead to improved source tracing and risk-based sampling.

Methods: We investigated the genomic epidemiology of in the Netherlands between 2010 and 2020 by analyzing whole-genome-sequencing (WGS) data of isolates from listerioss patients and food sources from nationwide integrated surveillance and monitoring.

The costs of chronic mastitis: A simulation study of an automatic milking system farm.

Mastitis is a production disease in dairy farming that causes economic losses. Especially chronic mastitis (i.e.

Sporadic Occurrence of Enteroaggregative Shiga Toxin-Producing Escherichia coli O104:H4 Similar to 2011 Outbreak Strain.

We describe the recent detection of 3 Shiga toxin-producing enteroaggregative Escherichia coli O104:H4 isolates from patients and 1 from pork in the Netherlands that were genetically highly similar to isolates from the 2011 large-scale outbreak in Europe. Our findings stress the importance of safeguarding food supply production chains to prevent future outbreaks.

Estimating the Effect of a Bovine Viral Diarrhea Virus Control Program: An Empirical Study on the Performance of Dutch Dairy Herds.

More and more European countries have implemented a bovine viral diarrhea virus (BVDV) control program. The economic effects of such programs have been evaluated in simulations, but empirical studies are lacking, especially in the final stage of the program. We investigated the economic (gross margin) and production effects (milk yield, somatic cell count, and calving interval) of the herds obtaining BVDV-free certification based on longitudinal annual accounting and herd performance data from Dutch dairy herds between 2014 and 2019, the final stages of the Dutch national BVDV-free program.

Estimating the nonlinear association of online somatic cell count, lactate dehydrogenase, and electrical conductivity with milk yield.

Reduction of milk yield is one of the principal components in the cost of mastitis. However, past research into the association between milk yield and mastitis indicators is limited. Past research has not been based on online or in-line daily measurements and has not fully explored nonlinearity and the thresholds at which milk yield starts to decrease.

Simulating the mechanics behind sub-optimal mobility and the associated economic losses in dairy production.

Hoof disorders and sub-optimal mobility (SOM) are economically important health issues in dairy farming. Although the dynamics of hoof disorders have an important effect on cow mobility, they have not been considered in previous simulation models that estimate the economic loss of SOM. Furthermore, these models do not consider the varying severities of SOM.

Assessing the generalisability of a multicentre qualitative dementia research: the experience and challenges faced by the MinD project in Europe.

: Generalisation of findings is an important aspect of research and essential for evidence-based practice. While generalisation is common in quantitative research, there is a lack of generalisability in qualitative research. This paper presents the experience and challenges faced by the Designing for People with Dementia (MinD) project in meeting the requirements to strengthen the generalisation of findings on the lived experience of people living with dementia and their engagement to co-create designs to empower their everyday living.

Invited review: Toward a common language in data-driven mastitis detection research.

Sensor technologies for mastitis detection have resulted in the collection and availability of a large amount of data. As a result, scientific publications reporting mastitis detection research have become less driven by approaches based on biological assumptions and more by data-driven modeling. Most of these approaches try to predict mastitis events from (combinations of) raw sensor data to which a wide variety of methods are applied originating from machine learning and classical statistical approaches.

The effect of new bovine viral diarrhea virus introduction on somatic cell count, calving interval, culling, and calf mortality of dairy herds in the Dutch bovine viral diarrhea virus-free program.

Bovine viral diarrhea virus (BVDV) infection has a major effect on the health of cows and consequently on herd performance. Many countries have implemented control or eradication programs to mitigate BVDV infection and its negative effects. These negative effects of BVDV infection on dairy herds are well documented, but there is much less information about the effects of new introduction of BVDV on dairy herds already participating in a BVDV control program.

Progression of different udder inflammation indicators and their episode length after onset of inflammation using automatic milking system sensor data.

In automatic milking systems (AMS), sensors can measure cow behavior and milk composition at every milking. The aim of this observational study of previously collected data was to gain insight into the differences in dynamics of udder inflammation indicators between cows that recover and those that do not recover after detection of an initial inflammation. Milk diversion (milk separated from the bulk tank and thus indicating farmer intervention), conductivity, and somatic cell count (SCC) data from 4 wk before the initial inflammation to 12 wk after the initial inflammation were used to analyze 2,584 cases of udder inflammation.

The effect of bovine viral diarrhea virus introduction on milk production of Dutch dairy herds.

Dairy cows are negatively affected by the introduction of bovine viral diarrhea virus (BVDV), and consequently, produce less milk. Existing literature on potential milk production losses is based on relatively outdated data and hardly evaluates milk production loss in relation to a new BVDV infection in a surveillance system. This study determined the annual and quarterly loss in milk production of BVDV introduction in 3,126 dairy herds participating in the Dutch BVDV-free program between 2007 and 2017.

Sources and transmission routes of campylobacteriosis: A combined analysis of genome and exposure data.

Objectives: To determine the contributions of several animal and environmental sources of human campylobacteriosis and identify source-specific risk factors.

Methods: 1417 Campylobacter jejuni/coli isolates from the Netherlands in 2017-2019 were whole-genome sequenced, including isolates from human cases (n = 280), chickens/turkeys (n = 238), laying hens (n = 56), cattle (n = 158), veal calves (n = 49), sheep/goats (n = 111), pigs (n = 110), dogs/cats (n = 100), wild birds (n = 62), and surface water (n = 253). Questionnaire-based exposure data was collected.

Draft Genome Sequence of Lipopeptide-Producing Strain Pseudomonas fluorescens DSM 11579 and Comparative Genomics with sp. Strain SH-C52, a Closely Related Lipopeptide-Producing Strain.

DSM 11579 is known to be a producer of the lipopeptides brabantamide and thanamycin. Its draft genome gives insight into the complete secondary metabolite production capacity of the strain and builds the basis for a comparative study with sp. strain SH-C52, a lipopeptide-producing strain involved in natural disease-suppressive soils.

Concordance of SNP- and allele-based typing workflows in the context of a large-scale international Enteritidis outbreak investigation.

A large European multi-country serovar Enteritidis outbreak associated with Polish eggs was characterized by whole-genome sequencing (WGS)-based analysis, with various European institutes using different analysis workflows to identify isolates potentially related to the outbreak. The objective of our study was to compare the output of six of these different typing workflows (distance matrices of either SNP-based or allele-based workflows) in terms of cluster detection and concordance. To this end, we analysed a set of 180 isolates coming from confirmed and probable outbreak cases, which were representative of the genetic variation within the outbreak, supplemented with 22 unrelated contemporaneous .

An international outbreak of Salmonella enterica serotype Enteritidis linked to eggs from Poland: a microbiological and epidemiological study.

Background: Salmonella spp are a major cause of food-borne outbreaks in Europe. We investigated a large multi-country outbreak of Salmonella enterica serotype Enteritidis in the EU and European Economic Area (EEA).

Methods: A confirmed case was defined as a laboratory-confirmed infection with the outbreak strains of S Enteritidis based on whole-genome sequencing (WGS), occurring between May 1, 2015, and Oct 31, 2018.