Publications by authors named "Volovikov E"
Astrocytes are subtypes of glial cells involved in metabolic, structural, homeostatic, and neuroprotective processes that help neurons maintain viability. Insulin-like growth factors IGF-1 and IGF-2 are known to have neuroprotective effects on neurons and glial cells through interaction with specific receptors. IGF forms a complex with IGF-binding proteins (IGFBP) in nervous tissue and is released from the complex via IGFBP proteolysis by specific proteases.
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- Pathogenic variants in the Gαo gene, crucial for brain function, are associated with severe neurological disorders, including developmental delays and epilepsy.
- A study utilized patient-derived induced pluripotent stem cells to investigate the effects of the p.G203R variant, discovering issues in cell development and differentiation into neurons, characterized by abnormal gene expression.
- Functional tests revealed that neurons with the p.G203R mutation exhibited lower calcium levels, reduced activity, and impaired responses to neurotransmitters, indicating a significant impact on brain signaling and development.
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- * The study investigated calcium signaling in GABA-ergic medium spiny neurons derived from iPSCs created through different methods (lentivirus and Sendai virus) from the same donor's fibroblasts.
- * Results showed no significant differences in calcium signaling between neurons from both reprogramming methods, indicating compatibility for combining data from different iPSC models and enhancing biobanking potential.
Parkinson's disease (PD) is a neurodegenerative pathology caused by the progressive loss of dopaminergic neurons in the substantia nigra. Juvenile PD is known to be strongly associated with mutations in the PARK2 gene encoding E3 ubiquitin ligase Parkin. Despite numerous studies, molecular mechanisms that trigger PD remain largely unknown.
View Article and Find Full Text PDFThe human induced pluripotent stem cell (iPSC) lines, iCS-MAF1-1 and iCS-MAF1-11, were generated from fibroblasts. The donor has a heterozygous mutation in the VPS13B gene, which manifests in her child as Cohen syndrome. It is a Golgi pathology, characterized by postnatal microcephaly and delayed growth and mental development.
View Article and Find Full Text PDFThe iPSC-derived brain organoid is a promising technology for in vitro modeling the pathologies of the nervous system and drug screening. This technology has emerged recently. It is still in its infancy and has some limitations unsolved yet.
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- * Research using induced pluripotent stem cell technology revealed that HD76 neurons experience abnormal calcium signaling, showing increased calcium uptake that does not correlate with the length of the mutant huntingtin gene's polyglutamine tract.
- * The study identified high levels of the protein STIM2, which is linked to excessive calcium entry in HD neurons, and found that the drug EVP4593 can reduce levels of both huntingtin and STIM2, highlighting STIM2 as a potential target for developing new treatments for HD
Organoids are three-dimensional (3D) cell cultures that replicate some of the key features of morphology, spatial architecture, and functions of a particular organ. Organoids can be generated from both adult and pluripotent stem cells (PSCs), and complex organoids can also be obtained by combining different types of cells, including differentiated cells. The ability of pluripotent cells to self-organize into organotypic structures containing several cell subtypes specific for a particular organ was used for creating organoids of the brain, eye, kidney, intestine, and other organs.
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