Physiological and Biochemical Responses of L. to Benzo[a]pyrene Contaminated Soils.

Benzo[a]pyrene (BaP) is noted as one of the main cancer-causing pollutants in human beings and may damage the development of crop plants. The present work was designed to explore more insights into the toxic effects of BaP on L. at various doses (20, 40, and 60 MPC) spiked in Haplic Chernozem.

Responses of Spring Barley to Zn- and Cd-Induced Stress: Morphometric Analysis and Cytotoxicity Assay.

Heavy metals such as cadmium (Cd) and zinc (Zn) could be dangerous and pollute the environment due to their high migration ability, robust bioavailability, and acute toxicity to soil biota and plants. Considering the above characteristics of these elements, the study's aim was to explore the individual and combined impact of Cd and Zn contamination of Haplic Chernozem on growing two-row spring barley ( L.).

Zinc Oxide Nanoparticles: Physiological and Biochemical Responses in Barley ( L.).

This work aimed to study the toxic implications of zinc oxide nanoparticles (ZnO NPs) on the physio-biochemical responses of spring barley ( L.). The experiments were designed in a hydroponic system, and was treated with two concentrations of ZnO NPs, namely 300 and 2000 mg/L.

Effect of ZnO Nanoparticles on Growth and Biochemical Responses of Wheat and Maize.

Zinc is an essential element that is also renowned for widespread contamination and toxicity at high concentrations. The present study was carried out to analyze the responses induced by lower, as well as higher, doses of zinc (0-200 mg/L), in the form of zinc oxide nanoparticles (ZnO NPs) in wheat and maize, for a period of 21 days. Accumulation of zinc increases with increasing Zn doses in both wheat and maize, with higher doses being in wheat (121 mg/kg in root and 66 mg/kg in shoot) than in maize (95 mg/kg in root and 48 mg/kg in shoot).

Potentially toxic elements in surface soils of the Lower Don floodplain and the Taganrog Bay coast: sources, spatial distribution and pollution assessment.

The pollution of floodplain, deltaic and adjacent coastal soils in large fluvial systems, considered an urgent environmental problem, as well as potentially toxic elements in such environments, can negatively affect aquatic ecosystems, as well as pose significant risks to human health. This paper is devoted to the geochemistry of potentially toxic elements in soils of the Lower Don basin, which is one of the largest and most anthropogenically transformed water bodies in Southern Russia, as well as the adjacent areas of the Taganrog Bay coast. The median element concentrations in the soils of the study area were consistent with the world soil average and the contents of elements in background soils.

Prevalence and genetic diversity of Wolbachia endosymbiont and mtDNA in Palearctic populations of Drosophila melanogaster.

Background: Maternally inherited Wolbachia symbionts infect D. melanogaster populations worldwide. Infection rates vary greatly.

[Molecular and genetic description of a new hypomorphic mutation of Trithorax-like gene and analysis of its effect on Drosophila melanogaster oogenesis].

The Trithorax-like (Trl) gene of Drosophila melanogaster encodes the multifunctional protein GAGA involved in many cellular processes. We have isolated and described a new hypomorphic mutation of the Trl gene--Trl(en82). The mutation is the insertion of a 1.

[The effect of genetic environment on locus-specific instability of the yellow gene in Uman' population of Drosophila melanogaster].

The effects of genotype of the laboratory strains, C(1)DX, ywf/Y, 23.5 MRF/CyL4, and C(1)DX,yf; pi2, on locus-specific instability in the yellow gene of the strains y(2-717, y(2-715), and y(2-700 ) from Uman' population of Drosophila melanogaster was studied. Crosses of the males from Uman'-derived lines with the C(1)DX, ywf/Y females yielded a cascade of derivatives, mostly consisting of y+ and y2 alleles, while their crosses with the 23.

[Behavior of hobo and P transposons in yellow2-717 unstable line of Drosophila melanogaster and its derivatives after crossing with a laboratory strain].

Using fluorescent in situ hybridization technique (FISH), the frequency of hobo and P mobile elements transpositions on X chromosomes from the y2-717, isolated from the Uman' population of Drosophila melanogaster, as well as from its phenotypically normal and mutant derivatives, obtained as a result of crosses the males examined with the C(I)DX, ywf/Y females, was evaluated. It was demonstrated that the maximum frequency of hobo transpositions on X chromosomes of the males from derivative strains, subjected to repeated hobo-dysgenic crosses reached a value of 1.2 x 10(-2) per site per X chromosome per generation.

[Cause for maintaining high instability at the yellow gene in Drosophila melanogaster lines, isolated during the "mode for mutation" period in Uman populations].

Mobile genetic elements are responsible for most spontaneous mutations in Drosophila melenogaster. The discovered in the 1980s phenomenon of frequent change of the wild-type yellow phenotype for a mutant one, and vice-versa, in strains of Drosophila melanogaster isolated from the Uman' natural population can be, according to our data, explained by repeated inversions and reinversions of the gene regulatory region located between the two copies of the hobo transport. However, most molecular genetic events accompanying the process can occur without the phenotype change.

["Mode for mutation" in the natural population of Drosophila melanogaster from Umani is caused by distribution of a hobo-induced inversion in the regulatory region of the yellow gene].

A mutation outburst of the yellow gene occurred in a Drosophila melanogaster population from the town of Uman' from 1982 to 1991 and was associated with the instability of several alleles. Molecular genetic analysis revealed a deletion variant of the hobo transposable element in the same site of the regulatory region of yellow in the mutant alleles and their derivatives. The outburst of the yellow-2 mutations was attributed to the spreading of the X chromosome, which contained an inversion of the yellow regulatory region, through the population.

hobo-induced rearrangements are responsible for mutation bursts at the yellow locus in a natural population of Drosophila melanogaster.

In 1981 recurrent local bursts of mutability of the yellow gene were observed in a natural population of Drosophila melanogaster from Uman' (Ukraine). A series of y2-like mutations in the yellow gene were recovered during the period 1982 to 1991. Most of the mutants display the y2-phenotype, i.

[Mutation bursts of the yellow gene in a natural Drosophila melanogaster population is connected with insertion of the hobo transposon].

In 1981, a recurrent local burst of high mutability and high allele frequency of the yellow gene was recorded in the natural population of Drosophila melanogaster from Uman', Ukraine. A detailed genetic analysis showed that hypomorphic alleles y2 prevailed during the mutation burst. Mutations were strictly allele-specific and occurred only in two directions: from y2 to y+ and from y+ to y2.

[Effect of genetic background on mutation frequency of insertional alleles of the lozenge in Drosophila melanogaster].

We studied the effect of genetic background on mutation frequency of an unstable lz75V allele of the lozenge gene (lz; 1-27.7) isolated from natural populations of Drosophila melanogaster and its mutant derivatives lzB abd lzsl. Genetic composition of the X chromosome containing unstable alleles (X75V chromosome) was shown to affect their mutability.

[Genetic instability in the Drosophila melanogaster lozenge locus: characteristics of the lz75V locus].

Genetic properties of lz75V, an unstable allele of the lozenge locus, are described. The lz75V allele appeared in progeny of a male from a Far East natural population of Drosophila melanogaster. Mutation of this allele produces a broad spectrum of mutant derivatives with phenotypes varying from normal to extreme.

[Preparation and primary genetic analysis of Drosophila melanogaster transformants line w'lz(b)/XXywf, containing mini-white genes, integrated in the genome during P-element-dependent transformation].

Transformation of Drosophila melanogaster using P-element-based vectors yielded 129 sublines, which carried mini-white gene copies in the different genome regions. Dependence of mini-white gene expression on the location, gene dosage, and sex of the transformed individuals was analyzed. The mutation lzb was shown to suppress mini-white gene expression, the degree of suppression depending on the location and dosage of the mini-white gene.

[The physicochemical anticomplement properties of alpha 2-macroglobulin for intravenous administration].

Stabilized composition of alpha-2-macroglobulin retains its physico-chemical and biological properties for 1 year storage at (6 +/- 2) degrees C. During this term it does not change its anticomplement activity, i.e.

[Efficacy of the use of a stabilized alpha2-macroglobulin concentrate in experimental burns].

The effectiveness of the use of a stabilized composition of alpha 2-macroglobulin concentrate obtained from fraction III of plasma proteins according to Cohn in burn disease in the experiment on guinea pigs was studied. Under the influence of intraabdominal administration for 4 days of alpha 2-macroglobulin concentrate with a stabilizer, healing of the burn wounds, normalization of a state of the animals occurred 2 times more rapidly than in control ones, activity of acid proteinases restored by day 5 (in control--by day 14) due to increase in activity of proteolytic enzyme inhibitors.

[Toxico-pharmacologic and genetic study of a novel cryopreserving solution for bone marrow cells].

Physico-chemical, biological and genetic properties of a cryopreserving solution for myelokaryocytes were studied. It was shown that the solution is harmless for the body, stable during storage, it produces no teratogenic or mutagenic effects, it should not be washed out before bone marrow cell administration to the recipient.

[Indicators of the blood coagulation system in dogs after compensation of blood loss with protesalin--a new plasma substitute solution].

It is shown that coagulability depression developed after acute hemorrhage (50 ml/kg) is not intensified by transfusion of the equal volume of protesalin, a new plasma-substituting solution. Normalization of the studied indices is observed a week after the blood loss and transfusion of the plasma-substituting solution.

[Interaction of mobile elements P and mdg3 in Drosophila melanogaster: genetic aspects].

It was found earlier that two unstable sn mutants isolated from natural populations are connected with insertion of mobile element mdg3 into the 7D1-2 region where singed gene (1-21.0) is localised. From two original sn mutants, a series of unstable sn alleles, both mutant and normal for phenotype, was extracted.