T-cadherin modulates adipogenic differentiation in mesenchymal stem cells: insights into ligand interactions.

Introduction: T-cadherin, a non-canonical member of the cadherin superfamily, was initially identified for its involvement in homophilic recognition within the nervous and vascular systems. Apart from its adhesive function, T-cadherin acts as a receptor for two ligands: LDL, contributing to atherogenic processes, and HMW adiponectin, a hormone with well-known cardiovascular protective properties. However, the precise role of T-cadherin in adipose tissue remains elusive.

Code-Free Machine Learning Solutions for Microscopy Image Processing: Deep Learning.

In recent years, there has been a significant expansion in the realm of processing microscopy images, thanks to the advent of machine learning techniques. These techniques offer diverse applications for image processing. Currently, numerous methods are used for processing microscopy images in the field of biology, ranging from conventional machine learning algorithms to sophisticated deep learning artificial neural networks with millions of parameters.

Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling.

Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, tissue renewal and regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g.

Practical Use of Immortalized Cells in Medicine: Current Advances and Future Perspectives.

In modern science, immortalized cells are not only a convenient tool in fundamental research, but they are also increasingly used in practical medicine. This happens due to their advantages compared to the primary cells, such as the possibility to produce larger amounts of cells and to use them for longer periods of time, the convenience of genetic modification, the absence of donor-to-donor variability when comparing the results of different experiments, etc. On the other hand, immortalization comes with drawbacks: possibilities of malignant transformation and/or major phenotype change due to genetic modification itself or upon long-term cultivation appear.

Decreased Insulin Sensitivity in Telomerase-Immortalized Mesenchymal Stem Cells Affects Efficacy and Outcome of Adipogenic Differentiation .

Modern biomedical science still experiences a significant need for easy and reliable sources of human cells. They are used to investigate pathological processes underlying disease, conduct pharmacological studies, and eventually applied as a therapeutic product in regenerative medicine. For decades, the pool of adult mesenchymal stem/stromal cells (MSCs) remains a promising source of stem and progenitor cells.

Hemochromatosis - modern condition of the problem.

The iron overload syndrome can be associated with various acquired states and hereditary factors. Hereditary hemochromatosis is the most common genetic disorder. Without therapeutic intervention the disease can lead to the development of life-threatening complications such as cirrhosis, hepatocellular carcinoma.

Operando XAS and UV-Vis Characterization of the Photodynamic Spiropyran-Zinc Complexes.

Thermal and photoinduced processes accompanying complexation of photochromic spiropyrans (SPP) with Zn ions in acetone solution were characterized by means of UV-vis and X-ray absorption spectroscopies in operando regime. SPP ligands are usually transparent at λ > 350 nm, but after mixing with Zn ions, they produce a stable colored (ε = 32 000-38 000 M cm) complex with maximum absorption at 525 nm, which makes them a powerful tool for monitoring metal-ion concentration in solution. The complex revealed fluorescence and photochromic behavior under static irradiation with visible light with constant photoreaction quantum yield across its characteristic absorption band.

Quantitative investigations of cation complexation of photochromic 8-benzothiazole-substituted benzopyran: towards metal-ion sensors.

The photochromic, thermochromic and metallochromic behaviour of a series of three spiro[indoline-8-(benzothiazol-2-yl)-benzopyrans] has been investigated. The thermodynamic and kinetic parameters of their thermal equilibrium between the ring-closed (spiro) and ring-opened (merocyanine) isomeric forms have been determined using UV-Vis absorption and (1)H NMR spectroscopies. By adding Co(ii) and Ni(ii) ions in acetonitrile solution, 1 : 1 and 1 : 2 metal : merocyanine complexes are formed simultaneously.

Convenient asymmetric synthesis of beta-substituted alpha,alpha-difluoro-beta-amino acids via Reformatsky reaction between Davis' N-sulfinylimines and ethyl bromodifluoroacetate.

The enantiopure Davis' N-sulfinylimines were found to be efficient as chiral imine equivalents in the high-temperature Reformatsky-type additions with BrZnCF(2)COOEt affording an efficient approach to the enantiomerically pure alpha,alpha-difluoro-beta-amino acids. High chemical and stereochemical yields (drs > 9:1, and as high as 99:1) render this method immediately useful for preparing the target amino acids.

[On mechanisms underlying bronchial obstruction in chronic dust bronchitis in workers of deep coal mines].

Healthy miners demonstrated increased relative levels of lysophosphatidyl choline and especially phosphatidyl inositol subunits. Metabolism of the latter could result in membrane accumulation of inositol triphosphate leading to cytoplasmic calcium increase and to bronchial smooth muscles contraction. Oral 20% choline chloride solution lowers bronchospasm in dust bronchitis patients with decreased serum subunits of phosphatidyl inositol and increased serum levels of phosphatidyl choline.

[Epithelial canaliculi of the thymus gland--thymosin-dependent structures].

The morphology peculiarities of thymus epithelial canals of 120 Wistar-line rats with thymosin have been observed. Thymosin-5 introduction during 3 days after birth in the dosage of 50 g proved to eliminate the epithelial canals of rat thymus in 3, 7 days of the postnatal period. In this period the epithelial canals have been found in thymus of all intact and control physiological solution injected rats.

[The role of the thymus medulla of newborn rats in lymphocytopoiesis].

By means of 3H-thymidine, radioautographic investigation of lymphocytopoiesis has been performed in the cortex and medulla of the rat thymus for 2 weeks after birth. In the newborn animals the index of labelled nuclei (ILN) in the medulla is higher than in the cortex. By the 14th day after birth a progressive drop of lymphocytes including the isotope takes place in the medulla.

[Status of the rat thymus after antenatal antigenic stimulation].

Morphological reactions of the thymus to penetration of foreign antigens into the organism should be further investigated. In the work presented, morphometric, histological and histochemical methods have been applied to study specific thymic reactions of the rat fetuses to antenatal antigenic stimulation. The antigenic stimulation of the fetuses results in an increased thymic function.