Spatiotemporal profiling of starch biosynthesis and degradation in the developing barley grain.

Barley (Hordeum vulgare) grains synthesize starch as the main storage compound. However, some starch is degraded already during caryopsis development. We studied temporal and spatial expression patterns of genes coding for enzymes of starch synthesis and degradation.

Distinct tubulin genes are differentially expressed during barley grain development.

Tubulins, as major components involved in the organization of microtubules, play an important role in plant development. We describe here the expression profiles of all known alpha-tubulin (TUA), beta-tubulin (TUB) and gamma-tubulin (TUG) genes of barley (Hordeum vulgare), involving eight newly identified TUB sequences, five established TUA genes and one TUG gene. Macroarray and Northern blot-based expression patterns in the pericarp, endosperm and embryo were obtained over the course of the development of the grain between anthesis and maturation.

The methylation cycle and its possible functions in barley endosperm development.

Barley endosperm development can be subdivided into the pre-storage, intermediate, storage and desiccation phase. Nothing is known about DNA methylation events involved in different endosperm-specific developmental programmes. A complete set of methylation cycle enzyme genes was identified and investigated by mRNA expression analysis.

Genetic transformation of cauliflower (Brassica oleracea var. botrytis) by direct DNA uptake into mesophyll protoplasts.

Mesophyll protoplasts of Brassica oleracea var. botrytis were successfully transformed using polyethylene glycol (PEG). The success of plant transformation depended on both gene transfer and plant regeneration.