Radical removal of a malignant mediastinum hemangioendothelioma with restoration of the main blood supply to the brain.

46 year old man appealed to the Cancer Research Center of RAMS in October 2012 with unverified anterior superior mediastinal tumor, which was diagnosed in 2010. Progressive compartment syndrome of the superior vena cava was observed. On examination: CT, MRI, angiography, histological and cytological examination of biopsy material did not allow to confirm the morphological structure of the tumor.

[Casein kinase 2, the versatile regulator of cell survival].

Casein kinase 2 (CK2), a highly conservative, multifunctional serine/threonine protein kinase, is critically important for the regulation of a plethora of processes in eukaryotes, such as cell proliferation, differentiation and death. CK2 is expressed in all tissues; in particular, its amount and activity are elevated in tumor cells. Unlike many regulatory proteins CK2 permanently adopts an active conformation.

[Morphological changes in esophageal mucosa in children with overweight].

In present work we studied the morphological features of the esophageal mucosa in 63 children with endoscopic diagnosis of the distal esophagitis having overweight and normal weight of a body. The biopsies were taken at level of 3 cm above a Z-line and at level of 1 cm above a Z-line. Dystrophic and dysregenerative changes were revealed at the majority of children and half of children had inflammatory changes of the esophageal mucosa regardless of weight of a body.

[Stimulation of differentiation of LIM 1215 large intestinal tumor cells during expression of the p53 exogenous antioncogene or the activated ras oncogene].

The ability of exogenous p53 tumor-suppressor and activated N-PAS oncogene to influence differentiation state of human colon carcinoma LIM 1215 cells and their derivatives with acquired resistance to actinomycin D or methotrexate was analysed Introduction of retroviral construct expressing human wild-type (wt) p53 into LIM 1215 cells induced electron microscopic manifestations of enterocytic differentiation, i.e. caused an increase in the numbers of cells with microvilli, desmosomes and glandular-like lumens.

[The differentiation of tumor cells of the human large intestine during the acquisition of multiple drug resistance].

By selection in the medium containing increasing actinomycin D concentrations two sublines with acquired multidrug resistance (MDR) caused by P-glycoprotein (P170) overproduction were isolated. The obtained cell lines as well as parent cells grow in vitro as morphologically organized aggregates, so-called organoids. Comparative electron microscopic study of sensitive and drug resistant organoids has shown that the development of MDR was accompanied by the enhancement of the tumour cell differentiation: the percentage of differentiated cells, the extent of their maturity, and the quantity of lumens were higher in MDR organoids than in parent cell line.

[Experience with the use of an immunoglobulin erythrocyte preparation for the early laboratory diagnosis of pseudotuberculosis].

An immunoglobulin erythrocytic preparation for the rapid detection of Yersinia pseudotuberculosis was developed. The preparation was shown to be specific and sufficiently sensitive (8 x 10(5) microbial cells/ml). The preparation was used for the examination of 102 patients; in 62 of these patients the diagnosis was confirmed by means of common laboratory and clinical methods.

[Blood flow dynamics in adjacent microvessels of the brain in hypertensive rats].

Significant alterations in the blood flow velocity, its amplitude and oscillations were found in cerebral cortex's microvessels of 5-12 mu diameter in normotensive and spontaneously hypertensive rats. Associated alterations of the blood flow shifted by a few seconds, were found in adjacent vessels. These alterations seem to be due to hemodynamic readjustments in proximal parts of the vascular net.

[Effect of the ion composition and osmotic properties of blood plasma on the erythrocytes of normotensive and hypertensive rats].

Size of the erythrocytes was shown to change depending on the osmolality of "own" plasma in normotensive rats (WKY). Size of the erythrocytes was unaltered in hypoosmotic (the H2O load) and enhanced in hyperosmotic (the NaCl load) and isoosmotic (the KCl load) media in hypertensive rats (SHR). These specific properties of the erythrocytes are supposed to be due to differences in the transport mechanisms of ion transition through membranes.

[Rate of blood flow in the microvessels of the brain of the rat with spontaneous arterial hypertension].

In normal and hypertensive rats, hematological indices were studied as well as the linear blood flow velocity in the microvessels with diameter 6-12 mu. Both groups of animals were shown to develop obvious oscillations of the blood velocity coinciding neither with heart beats nor with respiratory movements. In rats with spontaneous arterial hypertension, the mean velocity was 844 mu/sec and the amplitude of blood flow oscillations was 175 mu/sec which was higher by 35% and 33.

[Mechanism of development of erythrocytosis in spontaneous arterial hypertension in rats].

Rats with spontaneous hypertension versus normotensive rats showed the development of erythrocytosis, an intensification of the erythropoietic properties of the blood plasma and an increase of the 2,3-DPG concentration in the red blood cells. An enhanced production of erythropoietin, a humoral stimulator of erythropoiesis, seems to be one of the causes of the erythrocytosis development in spontaneously hypertensive rats. An increase in the 2,3-DPG concentration in the erythrocytes is an important physiological mechanism ensuring the appropriate oxygen supply to tissues.

[Differential traits and antigenic properties of the S- and R-forms of Neisseria meningitidis].

The authors demonstrated a marked dissociation of the Neisseria meningitis cultures and the capacity to their rapid change from S- to R-forms. Differential signs of the S- and R-forms are described. Differences in the degree of dissociation of the cultures depending on their origin were shown: strains isolated from the cerebrospinal fluid and the blood of patients with meningitis proved to be less dissociated, and those isolated from carriers--more dissociated.