Separation of interstitial and tubular cells of human testis by means of an anti-substance P-antiserum. Androstane-3 beta, 17 beta-diol is exclusively formed by seminiferous tubules.

Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) originated in the tubular compartment. The ratio 3 alpha/3 beta-diol was found to be higher (0.

The effect of short term treatment with cyproterone acetate or flutamide on the metabolism of 5 alpha-dihydrotestosterone in human testicular tissue.

Testicular tissue obtained from ten patients orchiectomized for prostatic cancer was incubated with [3H]5 alpha-dihydrotestosterone (DHT) in order to study the metabolic transformation into 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol) and 5 alpha-androstane-3 beta,17 beta-diol (3 beta-diol). Throughout 5 days before surgery four subjects were treated with cyproterone acetate (CA). To three patients flutamide (F) was administered for the same period of time.

Concentrations of unconjugated 5 alpha-androstane-3 alpha, 17 beta-diol and 5 alpha-androstane-3 beta, 17 beta-diol and their precursor in human testicular tissue. Comparison with testosterone, 5 alpha-dihydrotestosterone, estradiol-17 beta, and with steroid concentrations in human epididymis.

The concentrations of testosterone and its tissular metabolites were determined in testicular and epididymal tissue obtained from eleven male subjects (aged 65-85 years) after orchiectomy for prostatic cancer. The steroids were measured in different tissular compartments, i.e.

The effect of infusions of 5 alpha-dihydrotestosterone or estradiol-17 beta on the concentration of some steroids in the human testicular vein and artery.

The concentrations of testosterone, 5 alpha-dihydrotestosterone, 5 alpha-androstan-3 alpha, 17 beta-diol, 5 alpha-androstane-3 beta, 17 beta-diol, estradiol-17 beta and testosterone-glucosiduronate were measured in the plasma of the testicular vein and artery simultaneously with the estimation in peripheral venous and arterial plasma 60 min after an infusion of 3000 micrograms dihydrotestosterone (DHT) or estradiol (E2), respectively, in patients undergoing orchiectomy for prostatic cancer. The results were as follows; following infusion of DHT or E2, both steroids were completely metabolized by the testes. After DHT the testicular secretion of E2 was significantly reduced.

Ultrafiltrable plasma testosterone and 5 alpha-dihydrotestosterone as measured across the human testes.

It has been confirmed that within the human plexus pampiniformis a venous-arterial transfer of testosterone (T) (5.95%) and of dihydrotestosterone (DHT) (16.58%) occurs.

Unconjugated 5 alpha-androstan-3 alpha, 17 beta-diol and 5 alpha-androstane-3 beta, 17 beta-diol in normal and pathological human seminal plasma. Comparison with testosterone, 5 alpha-dihydrotestosterone and testosterone-glucosiduronate.

By means of specific RIA unconjugated 5 alpha-androstan-3 alpha, 17 beta-diol (3 alpha-diol) and 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) have been estimated for the first time in normal and pathological human seminal plasma. Testosterone, 5 alpha-dihydrotestosterone and testosterone-glucosiduronate (T, DHT, TG) have been determined simultaneously. On average a significant decrease of both diols was established in seminal plasma obtained from males suffering from oligozoospermia, azoospermia, asthenozoospermia and teratozoospermia.

The effect of the antiandrogen II alpha-hydroxyprogesterone on sebum production and cholesterol concentration of sebum.

II alpha-Hydroxyprogesterone, a synthetic antiandrogen of low toxicity, was applied topically. In short-term experiments the sebum production of the forehead in normal male subjects was reduced significantly. The cholesterol concentration of sebum decreased to a greater extent.

Comparison of steroid concentrations in venous and arterial blood across the human testis. Unconjugated 5 alpha-androstane-3 beta, 17 beta-diol: an important androgen metabolite of the human testicular-epididymal unit.

By means of specific radioimmunoassays unconjugated testosterone, 5 alpha-dihydrotestosterone, 5 alpha-androstane-3 alpha, 17 beta-diol, 5 alpha-androstane-3 beta, 17 beta-diol and estradiol-17 beta as well as testosterone-glucosiduronate were estimated in the plasma of the spermatic vein and artery simultaneously with the determination in peripheral venous and arterial plasma in ten male patients undergoing orchiectomy for prostatic cancer. The following results were obtained: A positive concentration gradient was found for all steroids. The testicular "secretion" of 5 alpha-androstane-3 beta, 17 beta-diol was established for the first time.

Unconjugated 5 alpha-androstan-3 alpha, 17 beta-diol and 5 alpha-androstane-3 beta, 17 beta-diol in human plasma as measured by radioimmunoassay without chromatography.

A radioimmunoassay (RIA) without chromatography for the determination of 5 alpha-androstan-3 alpha, 17 beta-diol and of 5 alpha-androstane-3 beta, 17 beta-diol in human plasma by using highly specific antisera against the 15 beta-carboxyethylmercapto-bovine serum albumin conjugates is described. It could be shown that the 13% cross reaction of the 5 alpha-androstane-3 beta, 17 beta-diol antiserum with pure androst-5-ene-3 beta, 17 beta-diol was negligibly low in plasma extracts. Sensitivity, accuracy, precision, and linearity revealed results which comply with the requisites of a reliable RIA.

Plasma testosterone glucosiduronate: a reliable indicator of female hyperandrogenism (idiopathic hirsutism and androgenic alopecia).

Plasma concentrations of testosterone glucosiduronate (TG) have been determined by direct radioimmunoassay in seventeen normal females, twenty females with idiopathic hirsutism, and in twelve female subjects with androgenetic alopecia without hirsutism, respectively. The corresponding mean concentrations (+/- SD) of TG were 0.47 +/- 0.

Short-term response of testosterone, dihydrotestosterone, 5 alpha-androstane-3 beta, 17 beta-diol, testosterone-glucosiduronate and estradiol-17 beta as measured in the spermatic vein of human male subjects after infusion of gonadotropins.

In 8 male subjects with normal testicular function, blood was drawn from the left spermatic vein and from the right cubital vein before and during an infusion of 4,000 IU human chorionic gonadotropin (hCG) (preceded by a bolus of 1,000 IU) or of 500 IU hCG plus human menopausal gonadotropin (hMG), respectively, within 30 min. Testosterone levels in the spermatic vein blood increased rapidly up to 518 or 215%, respectively. An increase of estradiol-17 beta and of 5 alpha-dihydrotestosterone concentrations in spermatic vein blood was observed only in 1 and 2 subjects, respectively.

The determination of 5alpha-androstane-3alpha, 17beta-diol in human plasma by radioimmunoassay.

Antibodies have been raised in rabbits against 3alpha, 17beta-dihydroxy-5alpha-androstane-6-0-carboxymethyloxime coupled with Cohn's fraction IV-4. The antiserum exhibited significant cross reactions with 5beta-androstane-3alph1, 17beta-diol, 5alpha-dihydrotestosterone, and testosterone. No cross reactions were observed with 5alpha-androstane-3beta,17beta-diol and 5-androstene-3beta,17beta-diol.