Electroretinographic Assessment of Inner Retinal Signaling in the Isolated and Superfused Murine Retina.

Purpose: Longer-lasting electroretinographic recordings of the isolated murine retina were initially achieved by modification of a phosphate-buffered nutrient solution originally developed for the bovine retina. During experiments with a more sensitive mouse retina, apparent model-specific limitations were addressed and improvements were analyzed for their contribution to an optimized full electroretinogram (ERG).

Material And Methods: Retinas were isolated from dark-adapted mice, transferred to a recording chamber and superfused with different solutions.