Publications by authors named "Vojtech Prusa"
Yeast plasma-membrane Na/H antiporters (Nha/Sod) ensure the optimal intracellular level of alkali-metal cations and protons in cells. They are predicted to consist of 13 transmembrane segments (TMSs) and a large hydrophilic C-terminal cytoplasmic part with seven conserved domains. The substrate specificity, specifically the ability to recognize and transport K cations in addition to Na and Li, differs among homologs.
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- The human Na+/H+ antiporter NHA2 (SLC9B2) is a protein that helps transport sodium or lithium in exchange for protons and is linked to various diseases.
- Researchers used a salt-sensitive strain of yeast to optimize and test various mutated forms of NHA2, focusing on their ability to transport ions, where they discovered a key proline residue (P246) significantly affects ion selectivity and transport function.
- The study found that alterations to the protein's N-terminus can enhance activity, and changes in specific residues can either inhibit or enhance transport, highlighting the potential for rapid screening of genetic variations affecting NHA2 function.