Effect of the pH value when treating concentrate protein with formaldehyde on protein protection in the rumen.

Nine samples of soybean extracted meal were treated by fumigation in plastic bag with 0.5 g formaldehyde/100 g crude protein, the pH ranging from 2.1 to 10.

[Effect of piracetam on passive avoidance learning in ethanol-treated mice].

Mice received pre- and postnatal drinking water containing 20% ethanol. Ethanol treatment significantly impaired learning, reflected by shorter latency to enter the dark compartment of the apparatus. This effect could be antagonized by the administration of twice daily 100 mg/kg piracetam for ten days.

[Heparin and the prevention of thromboembolism in surgery].

Heparin was introduced to medical prophylaxis against postoperative thromboembolic complications 50 years ago, following its discovery, structural elucidation, and first clinical use. Isolated subcutaneous application with and without control of coagulative data was followed by additional administration of dihydroergotamine. Results were thus substantially improved.

[Value of immunoperoxidase with monoclonal antibodies after dismounting of previously stained sections].

Immunoperoxidase using monoclonal antibodies may be applied to paraffin sections previously stained. The precise nature of cells found by routine staining may thus be investigated. It also enables, with small biopsies, immunohistochemical identification of a tissue zone lost by serial sectioning.

[Effectiveness and side effects of low-molecular weight heparin-dihydroergotamine in preventing thromboembolism in abdominal surgery].

Low-molecular heparin-dihydroergotamine had been applied once a day to 103 patients and heparin-dihydroergotamine twice a day to 97 patients as part of a double-blind, controlled, consecutive, and randomised clinical study, with 200 abdominal surgery patients being involved. High-risk patients above 40 years of age and potentially affected by thrombotically predisposing factors were almost equally represented in both groups. Evidence to postoperative thrombosis was phlebographically produced in either group.

Identification of intratissue sites for xenobiotic activation and detoxication.

Results of immunohistochemical and histochemical investigations on xenobiotic-metabolizing enzymes and aryl hydrocarbon hydroxylase activity have demonstrated that xenobiotic activation and detoxication do not occur uniformly throughout the liver, skin, respiratory tract, and pancreas, four tissues that are targets for the toxic actions of xenobiotics that are biotransformed into reactive metabolites. It has been shown that there can be significant differences in the levels and activities of xenobiotic-metabolizing enzymes among even morphologically similar cells, that an inducer can affect a specific xenobiotic-metabolizing enzyme to significantly different extents within different cells in a tissue, and that inducers of xenobiotic-metabolizing enzymes can alter differentially the extents to which different cells within a tissue participate in xenobiotic metabolism. These studies also have revealed that the route of administration of an inducer can affect significantly the induction of xenobiotic-metabolizing enzymes and aryl hydrocarbon hydroxylase activity within an organ such as the pancreas.

Assessment of short-term prognosis after variceal bleeding in patients with alcoholic cirrhosis by early measurement of portohepatic gradient.

Seventy-two consecutively admitted patients with biopsy-proven alcoholic cirrhosis and a bleeding episode endoscopically proven to originate from ruptured esophageal varices were studied. Hemodynamic assessment was performed within 48 hr of admission using the transjugular approach. Mean portal pressure was found to be significantly greater in the group of patients who died than in those who survived for 1 week, 2 weeks or 1 month after admission.

[The red blood cells in growing guinea pigs. (Cavia cobaya). II. Communication: erythropoiesis and red blood cells in the postnatal development period].

Bone-marrow smears of 175 guinea pigs aged 1-27 days and venous blood samples of 351 animals aged 1-25 days were prepared for cell counting. A significant increase of erythroblasts were found between life day 1 and 2; normoblasts increased in number synchronously with a decrease of erythroblasts after the 5th day. The percentage of the erythroid bone marrow increased from 10 to 14 during the developmental period.

[The red blood cells in growing guinea pigs (Cavia cobaya). I. Communication: pre- and perinatal changes in bone marrow and blood].

118 blood samples, 98 bone-marrow smears of guinea pig fetuses, 156 blood samples of spontaneously born guinea pigs 1 to 5 days of age, and 119 bone-marrow smears of the same age group were analysed quantitatively. The normoblasts show significant differences in pre- and early postnatal animals: a peak of the cell count is found in the late fetus which decreases immediately after birth. The prenatal development of the red blood picture is characterised by an increase of erythrocyte count and hematocrit and a decrease of cell size, and reticulocyte count.

[Embolization of esophageal varices by the transjugular route in severe digestive hemorrhage in cirrhotic patients. Prospective study of 83 patients].

A prospective study was undertaken to assess the results of embolization of esophageal varices by the transjugular approach, in 83 patients with advanced cirrhosis (65 p. 100 Child's class C) admitted with severe bleeding (mean of 10 units of blood per patient). Embolization was successfully completed in 65 patients (78 p.

[Diagnosis of undifferentiated tumors by means of monoclonal antibodies on paraffin sections].

The diagnostic value of 3 monoclonal antibodies applied on to routinely processed surgical biopsies was assessed. These antibodies were directed against keratin polypeptide (KL1), epithelial membrane antigen (DAKO-EMA) and leucocyte common antigen (DAKO-LC). First, using a three-step immunoperoxidase procedure, we determined the phenotype of well differentiated carcinomas (21 cases), non-Hodgkin's malignant lymphomas (44 cases), malignant histiocytoses (3 cases), melanomas (5 cases), sarcomas (6 cases) and miscellaneous tumors (16 cases).

Immunochemical studies on biosynthesis of rat plasma angiotensinogen and its regulation by cortisol.

Glucocorticosteroid hormones increase the level of rat plasma angiotensinogen by increasing its rate of synthesis. Two forms of plasma angiotensinogen have been purified differing with respect to molecular weight and affinity to concanavalin A. Immunochemical studies using antibodies raised against the separated forms of angiotensinogen revealed cross-reactivity with both antigens.

Measurement of the postruminal digestibility of crude protein by the bag technique in cows.

A new method has been developed which permits the crude protein digestibility of feedstuffs in the intestine of cattle to be measured with little effort in terms of samples and experimental work. It consists of welding 0.4 .

Localization of a cytochrome P-450 isozyme (cytochrome P-450 PB-B) and NADPH-cytochrome P-450 reductase in rat nasal mucosa.

Antibodies raised against cytochrome P-450 PB-B, the major phenobarbital-inducible isozyme of rat hepatic microsomal cytochrome P-450, and NADPH-cytochrome P-450 reductase (EC 1.6.2.

Extraction by lithium chloride of hydroxyproline-rich glycoproteins from intact cells of Chlamydomonas reinhardii.

A procedure has been developed to isolate and analyse the cell-wall glycoproteins of Chlamydomonas reinhardii. Under appropriate conditions, cell-wall glycoproteins can be quantitatively extracted from intact cells by aqueous LiCl. Although proteins and glycoproteins, which are presumably not related to the cell wall, are coextracted with the cell-wall subunits, these components can be readily identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as demonstrated by comparative analysis of LiCl-extracts from wild-type cells and the cell-wall-deficient mutant CW-15.

Macromolecules released into the culture medium during the vegetative cell cycle of the unicellular green alga Chlamydomonas reinhardii.

The culture medium of growing Chlamydomonas reinhardii cells contains hydroxyproline-rich glycoproteins, which are mainly liberated during release of the zoospores from the mother-cell wall. Pulse-labelling studies with [3H]proline and [35S]methionine have been performed in order to detect the protein components released by synchronously growing cells at different stages of the cell cycle. When either [3H]proline or [35S]methionine were applied during the phase of cell growth, radioactive label appeared in the released macromolecules after a lag period of 40 min, whereas incorporation into the insoluble part of the cell wall was delayed only by 20 min.

[Esophageal stenosis with tracheo-bronchial heterotopy. Apropos of 3 cases].

Three cases of esophageal stenosis with tracheo-bronchial remnants within the wall of the esophagus, near the cardia, are reported: two girls and one boy; in three, this anomaly contains cartilage remnants. After various procedures, esophagoplasty was necessary when the boy of the first case was fourteen. Two girls was cured by a Thal procedure with follow-up of five and four years respectively.

[Merkel cell carcinoma of the skin. Anatomoclinical, ultrastructural and immunohistochemical study of 14 cases].

The clinical and pathological features of 14 cases of Merkel cell carcinoma are reported. They commonly arise in the skin of elderly patients, particularly on the face and pelvis. They have a loco-regional aggressivity (nodal metastases in 4 cases) but some patients die with disseminated metastases (2 cases).

[Transport of urea nitrogen from the intestines into the stomach in dairy cows].

In two experiments with one dairy cow each the utilisation of urea-N after its ruminal or duodenal infusion was comparatively investigated on two crude protein levels and different urease activities in the rumen. The rations contained 9.6 (I) and 14.

[Comparison of 3 methods of recording esophageal pH in the diagnosis of gastroesophageal acid reflux].

The aim of this prospective work was to analyze the results of 3 methods of esophageal pH recording (i. e. short-term pH test, 3-hr postprandial recordings, and 12-hr nocturnal pH recording) in 47 patients with suspected gastroesophageal reflux.

[Effect of the content of crude plant protein in feed on the utilization of urea in dairy cattle. 4. Passage of amino acids through the duodenum].

Model investigations carried out by means of a re-entrant method at the duodenum with dairy cows and with energy-equivalent rations (600 EFU cattle/kg DM), which after the supplementation of 11 g urea/kg DM (150 g/animal and day) contained 13.8 (I), 16.7 (II) and 20.